pGK51 [pGSK51] (ATCC® 67278)

Organism: Escherichia coli (Migula) Castellani and Chalmers  /  Clone Type: Clone  /  Depositors: University of Texas Health Science Center, YM Kupersztoch, University of Texas Health Science Center

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Designations pGK51 [pGSK51]
Species Escherichia coli (Migula) Castellani and Chalmers
Depositors University of Texas Health Science Center, YM Kupersztoch, University of Texas Health Science Center
Applications
produces protein heat-labile enterotoxin
produces protein heat-stable enterotoxin
Vector
Construct size (kb): 3.596999883651733
Insert
DNA: genomic
Insert lengths(kb): 0.9110000133514404
Gene product: heat-stable enterotoxin [lt]
Target Gene: heat-labile enterotoxin, heat-stable enterotoxin
Insert Size (kb) 0.911
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.686, 0.911; EcoRI/HindIII/SstI--2.686, 0.505, 0.406.
Sonicated extracts of E. coli HB101 transformed with this construct express both ST and LTB immunoreactivity.
The 0.406 kb EcoRI/SstI fragment encodes the heat stable enterotoxin, and the 0.505 kb SstI/HindIII fragment encodes the heat labile enterotoxin.
The latter fragment, derived from EWD299 (ATCC 37218), includes the coding region for the B subunit and 3' transcription and translation stop signals, but lacks the ribosome binding site and sequences encoding the signal peptide.
The former fragment is at the 5' end and includes the complete coding sequence and 5' regulatory regions (transcription initiation and ribosome binding site) but not the 3' transcription and translation stop signals.
Classification Enterobacteriaceae, Escherichia
References

Guzman-Verduzco LM, Kupersztoch YM. Fusion of Escherichia coli heat-stable enterotoxin and heat-labile enterotoxin B subunit. J. Bacteriol. 169: 5201-5208, 1987. PubMed: 3312169

Guzman-Verduzco LM, Kupersztoch YM. Rectification of two Escherichia coli heat-stable enterotoxin allele sequences and lack of biological effect of changing the carboxy-terminal tyrosine to histidine. Infect. Immun. 57: 645-648, 1989. PubMed: 2643580

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