pcD-125 [pcD-human IL-4 (clone 125)] (ATCC® 67029)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: DNAX Research Institute, FD Lee, DNAX Research Institute

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Designations pcD-125 [pcD-human IL-4 (clone 125)]
GenBank Number

M13982

Species Homo sapiens, human
Depositors DNAX Research Institute, FD Lee, DNAX Research Institute
Applications
in suitable host, produces protein interleukin 4
Vector
Construct size (kb): 3.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 0.6000000238418579
Tissue: helper T-cell line 2F1
Gene product: interleukin 4 [IL4]
Target Gene: interleukin 4
Insert Size (kb) 0.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Shipped: Freeze dried Escherichia coli MC1061 recA-containing the plasmid.

Comments
Restriction digests of the clone give the following sizes (kb): Bam HI--2.8, 0.8; Eco RI--3.6; HindIII--3.6.
The insert contains the following unique restriction sites: NheI; EcoRV; PstI(although there are two sites in the vector); PvuII; EcoRI; BglI.
References

Lee F, et al. cDNA clones coding for human protein exxhibiting a broad cellular activity spectrum (human interleukin-4). US Patent 5,552,304 dated Sep 3 1996

Yokota T, et al. Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities. Proc. Natl. Acad. Sci. USA 83: 5894-5898, 1986. PubMed: 3016727

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