pcD-125 [pcD-human IL-4 (clone 125)] (ATCC® 67029)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: DNAX Research Institute, FD Lee, DNAX Research Institute

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Designations pcD-125 [pcD-human IL-4 (clone 125)]
GenBank Number

M13982

Species Homo sapiens, human
Depositors DNAX Research Institute, FD Lee, DNAX Research Institute
Applications
in suitable host, produces protein interleukin 4
Vector
Construct size (kb): 3.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 0.6000000238418579
Tissue: helper T-cell line 2F1
Gene product: interleukin 4 [IL4]
Target Gene: interleukin 4
Insert Size (kb) 0.600
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information

Shipped: Freeze dried Escherichia coli MC1061 recA-containing the plasmid.

Comments
Restriction digests of the clone give the following sizes (kb): Bam HI--2.8, 0.8; Eco RI--3.6; HindIII--3.6.
The insert contains the following unique restriction sites: NheI; EcoRV; PstI(although there are two sites in the vector); PvuII; EcoRI; BglI.
References

Lee F, et al. cDNA clones coding for human protein exxhibiting a broad cellular activity spectrum (human interleukin-4). US Patent 5,552,304 dated Sep 3 1996

Yokota T, et al. Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities. Proc. Natl. Acad. Sci. USA 83: 5894-5898, 1986. PubMed: 3016727

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