pFER2.3 (ATCC® 65904)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: J Groffen

Permits and Restrictions

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Designations pFER2.3
Species Homo sapiens, human
Depositors J Groffen
Vector
Construct size (kb): 5.0
Insert
DNA: genomic
Insert lengths(kb): 2.299999952316284
Gene product: fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) [FER]
Alleles: A1, A2
Insert Size (kb) 2.300
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.6, 2.2; PstI--3.4, 1.4; BglII--4.8; PvuII--2.4, 2.3; HindIII--3.2, 1.6; BamHI--4.8.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.1, 1.1; BglII--1.1; HindIII--1.6; SstI--1.8.
A repeat-free probe from a fragment located immediately 3' to the gene. Does not contain exons.
Enzymes(s) not detecting polymorphism: EcoRI, BglII, BamHI, HindIII, Asp700, TaqI, MspI, RsaI, PstI.
References

Bapat B, et al. The human tyrosine kinase gene (FER) detects an RFLP with BglI. Nucleic Acids Res. 19: 4310, 1991. PubMed: 1678513

Morris C, et al. The human tyrosine kinase gene (FER) maps to chromosome 5 and is deleted in myeloid leukemias with a del(5q). Cytogenet. Cell Genet. 53: 196-200, 1990. PubMed: 2209086

John Groffen, personal communication

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