pFER2.3 (ATCC® 65904)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: J Groffen

Permits and Restrictions

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Designations pFER2.3
Species Homo sapiens, human
Depositors J Groffen
Vector
Construct size (kb): 5.0
Insert
DNA: genomic
Insert lengths(kb): 2.299999952316284
Gene product: fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) [FER]
Alleles: A1, A2
Insert Size (kb) 2.300
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.6, 2.2; PstI--3.4, 1.4; BglII--4.8; PvuII--2.4, 2.3; HindIII--3.2, 1.6; BamHI--4.8.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.1, 1.1; BglII--1.1; HindIII--1.6; SstI--1.8.
A repeat-free probe from a fragment located immediately 3' to the gene. Does not contain exons.
Enzymes(s) not detecting polymorphism: EcoRI, BglII, BamHI, HindIII, Asp700, TaqI, MspI, RsaI, PstI.
References

Bapat B, et al. The human tyrosine kinase gene (FER) detects an RFLP with BglI. Nucleic Acids Res. 19: 4310, 1991. PubMed: 1678513

Morris C, et al. The human tyrosine kinase gene (FER) maps to chromosome 5 and is deleted in myeloid leukemias with a del(5q). Cytogenet. Cell Genet. 53: 196-200, 1990. PubMed: 2209086

John Groffen, personal communication

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