p146-5 (ATCC® 65819)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations p146-5
Species Homo sapiens, human
Construct size (kb): 3.5
DNA: genomic
Insert lengths(kb): 0.5
Tissue: placenta St
Gene product: immunoglobulin kappa variable region [IGKV]
Alleles: Gd, Nd, Gd, Gd, Gd, Gp, Nd, Nd, Np, Np, Np, Gp, Gp, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Np, Gp, Np, Gp, Np, Gp, Np, Gp
Insert Size (kb) 0.5
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 0.5; PvuII--2.5, 0.6, 0.4; PvuI--2.4, 1.1; PvuI/EcoRI--1.7, 1.1, 0.7; PvuII/XbaI--2.5, 0.4, 0.3, 0.2, 0.2.
Detects sequences from the Op (between O18 and A15) and Od (between O8 and O9) regions.
In the AccI/BamHI digests of genomic DNA, the AccI site is polymorphic.
Recloned from m146-5. The insert contains a PvuII site approximately 0.1 kb from the HindII end.

Pargent W, et al. Polymorphisms and haplotypes in the human immunoglobulin kappa locus. Eur. J. Immunol. 21: 1829-1835, 1991. PubMed: 1678350

Pargent W, et al. The human immunoglobulin kappa locus. Characterization of the duplicated O regions. Eur. J. Immunol. 21: 1821-1827, 1991. PubMed: 1907917

Hans G Zachau, personal communication

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