JH-4 [G4-2, lambda-EZZ-gamma3] (ATCC® 65714)

Clone Type: Clone  /  Depositors: H Donis-Keller, JR Howe

Permits and Restrictions

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Designations JH-4 [G4-2, lambda-EZZ-gamma3]
Depositors H Donis-Keller, JR Howe
Vector
Construct size (kb): 47.70000076293945
Insert
DNA: genomic
Insert lengths(kb): 18.5
Gene product: retinol-binding protein 3, interstitial(retinol-binding protein 3, interstitial (includes former D10S64, D10S65, D10S66)) [RBP3]
Alleles: A2, B2, A1, A2, A2, A2, B2, B2, B2, A1, A1, A1, A1, A1, A1, A2, A2, A2, B1, B1, B2, C1, D1, D2, A1, A2, A3, C2, A1, A1, A1, A1, A1, A1, A1, A1, A1, A2, A2, A2, A2, A2, A2, A2, A2, A2, B1, B1, B1, B1, B1, B1, B2, B2, B2, C1, C1, C1, D1, D1, D1, D2, D2, D2, A1, A1, A1, A2, A2, A2, A3, A3, A3, C2, C2, C2
Insert Size (kb) 18.5
Biosafety Level 1
Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): SalI--20.0, 18.0, 9.2; SalI/BamHI--20.0, 12.5, 9.2, 4.4, 1.6; SmaI--20.0, 12.0, 7.8, 6.0, 1.8; HindIII--23.0, 6.0, 4.4.
Restriction digests of the clone give the following sizes (kb): BamHI/SalI--12.5, 4.4, 1.6 (insert), 20.0, 9.2 (vector).
The insert contains a MluI and a SacII site.
Subcloned from YAC IR244.
Enzyme(s) not detecting polymorphism: BamHI, BglII, HincII, HindIII (in a panel of 6 individuals).
References

Howe JR, et al. New YAC-derived polymorphic markers linked to the MEN-IIa locus on chromosome 10. Am. J. Hum. Genet. 47: A184, 1990.

Lairmore TC, et al. Familial medullary thyroid carcinoma and multiple endocrine neoplasia type 2B map to the same region of chromosome 10 as multiple endocrine neoplasia type 2A. Genomics 9: 181-192, 1991. PubMed: 1672289

Helen Donis-Keller, personal communication

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