JH-4 [G4-2, lambda-EZZ-gamma3] (ATCC® 65714)

Clone Type: Clone  /  Depositors: H Donis-Keller, JR Howe

Permits and Restrictions

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Designations JH-4 [G4-2, lambda-EZZ-gamma3]
Depositors H Donis-Keller, JR Howe
Vector
Construct size (kb): 47.70000076293945
Insert
DNA: genomic
Insert lengths(kb): 18.5
Gene product: retinol-binding protein 3, interstitial(retinol-binding protein 3, interstitial (includes former D10S64, D10S65, D10S66)) [RBP3]
Alleles: A2, B2, A1, A2, A2, A2, B2, B2, B2, A1, A1, A1, A1, A1, A1, A2, A2, A2, B1, B1, B2, C1, D1, D2, A1, A2, A3, C2, A1, A1, A1, A1, A1, A1, A1, A1, A1, A2, A2, A2, A2, A2, A2, A2, A2, A2, B1, B1, B1, B1, B1, B1, B2, B2, B2, C1, C1, C1, D1, D1, D1, D2, D2, D2, A1, A1, A1, A2, A2, A2, A3, A3, A3, C2, C2, C2
Insert Size (kb) 18.5
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): SalI--20.0, 18.0, 9.2; SalI/BamHI--20.0, 12.5, 9.2, 4.4, 1.6; SmaI--20.0, 12.0, 7.8, 6.0, 1.8; HindIII--23.0, 6.0, 4.4.
Restriction digests of the clone give the following sizes (kb): BamHI/SalI--12.5, 4.4, 1.6 (insert), 20.0, 9.2 (vector).
The insert contains a MluI and a SacII site.
Subcloned from YAC IR244.
Enzyme(s) not detecting polymorphism: BamHI, BglII, HincII, HindIII (in a panel of 6 individuals).
References

Howe JR, et al. New YAC-derived polymorphic markers linked to the MEN-IIa locus on chromosome 10. Am. J. Hum. Genet. 47: A184, 1990.

Lairmore TC, et al. Familial medullary thyroid carcinoma and multiple endocrine neoplasia type 2B map to the same region of chromosome 10 as multiple endocrine neoplasia type 2A. Genomics 9: 181-192, 1991. PubMed: 1672289

Helen Donis-Keller, personal communication