JH-2 [G3-3] (ATCC® 65710)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: H Donis-Keller, JR Howe

Permits and Restrictions

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Designations JH-2 [G3-3]
Species Homo sapiens, human
Depositors H Donis-Keller, JR Howe
Vector
Construct size (kb): 52.0
Insert
DNA: genomic
Insert lengths(kb): 22.79999923706055
Gene product: retinol-binding protein 3, interstitial(retinol-binding protein 3, interstitial (includes former D10S64, D10S65, D10S66)) [RBP3]
Alleles: A2, B2, A1, A2, A2, A2, B2, B2, B2, A1, A1, A1, A1, A1, A1, A2, A2, A2, B1, B1, B2, C1, D1, D2, A1, A2, A3, C2, A1, A1, A1, A1, A1, A1, A1, A1, A1, A2, A2, A2, A2, A2, A2, A2, A2, A2, B1, B1, B1, B1, B1, B1, B2, B2, B2, C1, C1, C1, D1, D1, D1, D2, D2, D2, A1, A1, A1, A2, A2, A2, A3, A3, A3, C2, C2, C2
Insert Size (kb) 22.800
Biosafety Level 1
Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): SalI--20.0, 9.2, 7.6, 7.0; BamHI/SalI--20.0, 9.2, 6.6, 3.8, 3.1, 1.0; HindIII--22.0, 12.0, 4.2, 2.8, 2.1, 0.6; KpnI--18.0, 16.0, 7.6, 1.7, 1.5; SmaI--20.0, 14.0, 6.0, 4.4.
Restriction digests of the clone give the following sizes (kb): BamHI/SalI--6.6, 3.8, 3.1, 1.0 (insert), 20.0, 9.2 (vector).
The insert contains a SalI site.
Detects the same polymorphism as cTBIRBP-9 (ATCC 59986-59987).
Detection of the TaqI polymorphism was verified.
Subcloned from YAC IR244.
Enzyme(s) not detecting polymorphism: BamHI, BglII, EcoRI, HindIII, MspI, PstI (in a panel of 6 individuals).
References

Howe JR, et al. New YAC-derived polymorphic markers linked to the MEN-IIa locus on chromosome 10. Am. J. Hum. Genet. 47: A184, 1990.

Helen Donis-Keller, personal communication

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