lambdaSH6 (ATCC® 65670)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: M Lefranc, TH Rabbitts

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Designations lambdaSH6
GenBank Number

X08084

Species Homo sapiens, human
Depositors M Lefranc, TH Rabbitts
Vector
Construct size (kb): 47.0
Insert
DNA: genomic
Insert lengths(kb): 17.5
Tissue: B lymphocyte (EBV transformed), SH cell line
Gene product: T cell receptor, gamma, joining segment JP1 [TCRGJ1]
Insert Size (kb) 17.5
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): KpnI--18.0, 9.8, 8.8, 7.5, 1.5; PvuI--23.0, 12.5, 7.6; SacI--20.0, 9.0, 5.6, 4.4, 3.3, 2.8, 1.2; SmaI--20.0, 16.0, 5.4 (doublet); XhoI--20.0, 9.2, 8.8, 5.2, 3.5.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--2.8, 8.6, 9.7, 14.1, 17.5 (linker site).
Contains the genes in the germ line configuration, beginning about 7.6 kb upstream of JP1 and extending about 3 kb beyond J1.
Additional GenBank/EMBL accession numbers: M12950, M12960.
References

Lefranc MP, et al. Molecular mapping of the human T cell receptor gamma (TRG) genes and linkage of the variable and constant regions. Eur. J. Immunol. 19: 989-994, 1989. PubMed: 2526744

Marie-Paule Lefranc, personal communication

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