lambdaSH6 (ATCC® 65670)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: M Lefranc, TH Rabbitts

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Designations lambdaSH6
GenBank Number

X08084

Species Homo sapiens, human
Depositors M Lefranc, TH Rabbitts
Vector
Construct size (kb): 47.0
Insert
DNA: genomic
Insert lengths(kb): 17.5
Tissue: B lymphocyte (EBV transformed), SH cell line
Gene product: T cell receptor, gamma, joining segment JP1 [TCRGJ1]
Insert Size (kb) 17.5
Biosafety Level 1
Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): KpnI--18.0, 9.8, 8.8, 7.5, 1.5; PvuI--23.0, 12.5, 7.6; SacI--20.0, 9.0, 5.6, 4.4, 3.3, 2.8, 1.2; SmaI--20.0, 16.0, 5.4 (doublet); XhoI--20.0, 9.2, 8.8, 5.2, 3.5.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--2.8, 8.6, 9.7, 14.1, 17.5 (linker site).
Contains the genes in the germ line configuration, beginning about 7.6 kb upstream of JP1 and extending about 3 kb beyond J1.
Additional GenBank/EMBL accession numbers: M12950, M12960.
References

Lefranc MP, et al. Molecular mapping of the human T cell receptor gamma (TRG) genes and linkage of the variable and constant regions. Eur. J. Immunol. 19: 989-994, 1989. PubMed: 2526744

Marie-Paule Lefranc, personal communication

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