cCJ52.199 (ATCC® 65092)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: C Julier

Permits and Restrictions

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Designations cCJ52.199
Species Homo sapiens, human
Depositors C Julier
Vector
Construct size (kb): 48.0
Insert
DNA: genomic
Insert lengths(kb): 40.0
Gene product: DNA Segment, single copy [D16S155]
Alleles: A1, A2
Insert Size (kb) 40.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: frozen
Comments
Restriction digests of the clones give the following sizes (kb): BamHI--15.0, 10.0, 8.6 (doublet), 4.1, 3.1, 2.4, 1.8, 1.0; HindIII--19.0, 15.0, 5.0, 4.5, 3.8, 3.6, 3.5; EcoRI--23.0, 8.6, 6.0, 5.2, 2.6, 2.5, 2.3, 2.1, 0.9.
BglII--9.4 (doublet), 6.5, 5.4, 5.1, 3.4, 2.4, 2.3, 1.6, 1.4, 1.35, 1.2, 1.0, 0.7, 0.6; NotI--42.0, 8.0.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Enzyme(s) not detecting polymorphism: TaqI, PstI.
References

Julier C, et al. A primary map of 24 loci on human chromosome 16. Genomics 6: 419-427, 1990. PubMed: 2328986

Julier C, et al. Isolation and mapping of a polymorphic DNA sequence (CJ52.199) on chromosome 16 [D16S155]. Nucleic Acids Res. 17: 8020, 1989. PubMed: 2477808

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