cCJ52.199 (ATCC® 65092)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: C Julier

Permits and Restrictions

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Designations cCJ52.199
Species Homo sapiens, human
Depositors C Julier
Vector
Construct size (kb): 48.0
Insert
DNA: genomic
Insert lengths(kb): 40.0
Gene product: DNA Segment, single copy [D16S155]
Alleles: A1, A2
Insert Size (kb) 40.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen
Comments
Restriction digests of the clones give the following sizes (kb): BamHI--15.0, 10.0, 8.6 (doublet), 4.1, 3.1, 2.4, 1.8, 1.0; HindIII--19.0, 15.0, 5.0, 4.5, 3.8, 3.6, 3.5; EcoRI--23.0, 8.6, 6.0, 5.2, 2.6, 2.5, 2.3, 2.1, 0.9.
BglII--9.4 (doublet), 6.5, 5.4, 5.1, 3.4, 2.4, 2.3, 1.6, 1.4, 1.35, 1.2, 1.0, 0.7, 0.6; NotI--42.0, 8.0.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Enzyme(s) not detecting polymorphism: TaqI, PstI.
References

Julier C, et al. A primary map of 24 loci on human chromosome 16. Genomics 6: 419-427, 1990. PubMed: 2328986

Julier C, et al. Isolation and mapping of a polymorphic DNA sequence (CJ52.199) on chromosome 16 [D16S155]. Nucleic Acids Res. 17: 8020, 1989. PubMed: 2477808

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