ml665 (ATCC® 63281)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: AJ Lusis

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Designations ml665
GenBank Number

L11812

L11813

M25529

Species Mus musculus, mouse
Depositors AJ Lusis
Vector
Construct size (kb): 3.849999904632568
Insert
DNA: cDNA
Insert lengths(kb): 0.8999999761581421
Tissue: liver
Gene product: DNA segment, Chr 12, University of California at Los Angeles-2 [Aat]
Alleles: B, S, ,
Insert Size (kb) 0.900
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.2; ClaI--3.0, 1.2; EcoRI--3.0, 1.2; PstI--3.5, 0.8; SacI--3.9, 0.35.
The insert contains the following restriction sites (approximate kb from the T3 primer end): BamHI--0.06; ClaI--0.12, 0.86; EcoRI--0.08; NotI--0.04; PstI--0.07, 0.10, 0.89; SacI--0.03; XbaI--0.05.
The sequences recorded in L11812 and L11813 appear to be from the same primer and offset by 60 nt.
Based on the insert size and the alignment with GenBank accession M25529 (1334 nt, CDS 9-1250), this clone appears to contain part of the coding sequence.
Detects a 2.3 kb transcript in the mouse heart.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Warden CH, et al. Linkage mapping of 40 randomly isolated liver cDNA clones in the mouse. Genomics 18: 295-307, 1993. PubMed: 8288233

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