ml812 (ATCC® 63259)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: AJ Lusis

Permits and Restrictions

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Designations ml812
GenBank Number

J05224

L11804

L11805

Species Mus musculus, mouse
Depositors AJ Lusis
Vector
Construct size (kb): 4.449999809265137
Insert
DNA: cDNA
Insert lengths(kb): 1.5
Tissue: liver
Gene product: aconitase-2, mitochondrial [Aco2]
Insert Size (kb) 1.5
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 0.9, 0.7; BglI--1.7, 1.3 (doublet), 0.3; PvuI--3.2, 1.1, 0.2; XbaI--3.9, 0.55; PvuII--2.4, 1.4, 0.7.
The insert contains the following restriction sites (approximate kb from the T3 primer end): BglI--0.14, 0.19; PvuI--1.49; XbaI--0.02.
Based on the alignment with J05224 for swine mitochondrial ac onitase (2665 nt, CDS 30-2375, approximately 80% identity over the regions sequenced), this clone appears to contain coding sequence.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Warden CH, et al. Linkage mapping of 40 randomly isolated liver cDNA clones in the mouse. Genomics 18: 295-307, 1993. PubMed: 8288233

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