pKLy6.1-2R (ATCC® 63181)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: AL Bothwell

Permits and Restrictions

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Designations pKLy6.1-2R
GenBank Number

X04653

Species Mus musculus, mouse
Depositors AL Bothwell
Vector
Construct size (kb): 3.5
Insert
DNA: cDNA
Insert lengths(kb): 0.7609999775886536
Tissue: MethA fibrosarcoma cell line
Gene product: lymphocyte antigen-6 [DAG]
Alleles: 6.1, 6.1, 6.1, 6.2, 6.2, 6.2, 6.1, 6.1, 6.2, 6.2, 6.1, 6.2
Insert Size (kb) 0.761
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BglI--2.3, 1.15; EcoRI--2.7, 0.8; PstI--3.2, 0.35; PvuII--2.4, 1.15; SacI--2.9, 0.3 (doublet).
The insert contains the following restriction sites (nt from the 5' end): SacI--160, 467; PstI--480.
Encodes Ly-6E.1 (Ly-6a allele) recognized by the SK70.94 and HD42 monoclonal antibodies.
Contains the complete coding sequence (nt 89-492) and extends from a linker-derived EcoRI site at the 5' end to a naturally occurring EcoRI site in the 3' untranslated region.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

LeClair KP, et al. Isolation of a murine Ly-6 cDNA reveals a new multigene family. EMBO J. 5: 3227-3234, 1986. PubMed: 3028776