pZP3.2 (ATCC® 63163)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: J Dean

Permits and Restrictions

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Designations pZP3.2
GenBank Number

M20026

Species Mus musculus, mouse
Depositors J Dean
Applications
Analysis of the sequence record predicts the following restriction sites (nt from the 5' end): SmaI--186, 223; PstI--609, 950; PvuII--612; BalI--634, 1165; BglI--1167.
Vector
Construct size (kb): 5.0
Insert
DNA: cDNA
Insert lengths(kb): 1.230000019073486
Tissue: oocyte
Gene product: zona pellucida glycoprotein 3( zona pellucida glycoprotein 3A, zona pellucida glycoprotein 3B, ZP3, glycoprotein 3, zona pellucida, zona pellucida protein C) [Zp3]
Alleles: s, b
Insert Size (kb) 1.230
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 1.3; PstI--3.4, 1.2, 0.4; BamHI--5.0; KpnI--5.0; BglI--2.2, 1.8, 1.0.
Analysis of the sequence record predicts the following restriction sites (nt from the 5' end): SmaI--186, 223; PstI--609, 950; PvuII--612; BalI--634, 1165; BglI--1167.
A nearly full-length cDNA, extending from nt 47-1275 (the coding region of the precursor extends from 30-1301 and the coding region of the predicted mature peptide extends from 96-1301).
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Lunsford RD, et al. Genomic mapping of murine Zp-2 and Zp-3, two oocyte-specific loci encoding zona pellucida proteins. Genomics 6: 184-187, 1990. PubMed: 1968044

Ringuette MJ, et al. Molecular analysis of cDNA coding for ZP3, a sperm binding protein of the mouse zona pellucida. Dev. Biol. 127: 287-295, 1988. PubMed: 3378665

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