pZP3.2 (ATCC® 63163)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: J Dean

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Designations pZP3.2
GenBank Number

M20026

Species Mus musculus, mouse
Depositors J Dean
Applications
Analysis of the sequence record predicts the following restriction sites (nt from the 5' end): SmaI--186, 223; PstI--609, 950; PvuII--612; BalI--634, 1165; BglI--1167.
Vector
Construct size (kb): 5.0
Insert
DNA: cDNA
Insert lengths(kb): 1.230000019073486
Tissue: oocyte
Gene product: zona pellucida glycoprotein 3( zona pellucida glycoprotein 3A, zona pellucida glycoprotein 3B, ZP3, glycoprotein 3, zona pellucida, zona pellucida protein C) [Zp3]
Alleles: s, b
Insert Size (kb) 1.230
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 1.3; PstI--3.4, 1.2, 0.4; BamHI--5.0; KpnI--5.0; BglI--2.2, 1.8, 1.0.
Analysis of the sequence record predicts the following restriction sites (nt from the 5' end): SmaI--186, 223; PstI--609, 950; PvuII--612; BalI--634, 1165; BglI--1167.
A nearly full-length cDNA, extending from nt 47-1275 (the coding region of the precursor extends from 30-1301 and the coding region of the predicted mature peptide extends from 96-1301).
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Lunsford RD, et al. Genomic mapping of murine Zp-2 and Zp-3, two oocyte-specific loci encoding zona pellucida proteins. Genomics 6: 184-187, 1990. PubMed: 1968044

Ringuette MJ, et al. Molecular analysis of cDNA coding for ZP3, a sperm binding protein of the mouse zona pellucida. Dev. Biol. 127: 287-295, 1988. PubMed: 3378665

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