6c (ATCC® 63128)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: M Chu

Permits and Restrictions

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Designations 6c
GenBank Number

X14480

Species Mus musculus, mouse
Depositors M Chu
Vector
Construct size (kb): 4.5
Insert
DNA: cDNA
Insert lengths(kb): 1.5
Tissue: Engelbreth-Holm-Swarm (EHS) tumor
Gene product: nidogen [Nid]
Alleles: B, S
Insert Size (kb) 1.5
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.5; BamHI--4.5; HindIII--4.5; PvuI--3.5, 1.0.
Restriction digests of the clone give the following sizes (kb): PvuII--2.8, 0.9, 0.8.
Contains a PstI site (nt 2627 of the published sequence) which is approximately 600 bp from the 5' EcoRI site.
Corresponds to sequences beginning about 1.5 kb upstream of the EcoRI site at nt 3525 of the published sequence, and encodes most of the rod-like domain and part of the C-terminal globular domain.
Detects mRNA of 6.0 and 4.0 kb (weak) in the mouse and 6.0 kb in the human.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Jenkins NA, et al. Nidogen/entactin (Nid) maps to the proximal end of mouse chromosome 13 linked to beige (bg) and identifies a new region of homology between mouse and human chromosomes. Genomics 9: 401-403, 1991. PubMed: 1672300

Mann K, et al. Amino acid sequence of mouse nidogen, a multidomain basement membrane protein with binding activity for laminin, collagen IV and cells. EMBO J. 8: 65-72, 1989. PubMed: 2496973

Mon-Li Chu, personal communication

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