pmP3450-3' (ATCC® 63007)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: DW Nebert

Permits and Restrictions

View Permits

Designations pmP3450-3'
Species Mus musculus, mouse
Depositors DW Nebert
Vector
Construct size (kb): 5.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 1.299999952316284
Tissue: liver polysomes
Gene product: cytochrome P450, 1a2, aromatic carbon inducible [P4503]
Alleles: a, b, a, b
Insert Size (kb) 1.300
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--4.4, 1.3; BamHI--5.6; HindIII--5.6; EcoRI--5.6; PstI--4.3, 1.0.
Murine P3-450 corresponds to rat P-450d and rabbit form 4.
The insert extends from the HindIII site at nt 579 through the poly(A) sequence.
Exhibits 29% nucleotide homology with rat P-450e; the protein homology is 22% with rat P-450e and 77% with rabbit form 2 protein.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Hildebrand CE, et al. Regional linkage analysis of the dioxin-inducible P-450 gene family on mouse chromosome 9. Biochem. Biophys. Res. Commun. 130: 396-406, 1985. PubMed: 4040754

Kimura S, et al. The murine Ah locus. Comparison of the complete cytochrome P1-450 and P3-450 cDNA nucleotide and amino acid sequences. J. Biol. Chem. 259: 10705-10713, 1984. PubMed: 6547952

Prochazka M, et al. Three recessive loci required for insulin-dependent diabetes in nonobese diabetic mice. Science 237: 286-289, 1987. PubMed: 2885918

Dey A, et al. Cell-specific induction of mouse Cyp1a1 mRNA during development. Proc. Natl. Acad. Sci. USA 86: 7446-7450, 1989. PubMed: 2477842

Daniel W Nebert, personal communication

Product Sheet
Product Sheet
Product Sheet