pmP3450-3' (ATCC® 63007)

Organism: Mus musculus, mouse  /  Clone Type: Clone  /  Depositors: DW Nebert

Permits and Restrictions

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Designations pmP3450-3'
Species Mus musculus, mouse
Depositors DW Nebert
Vector
Construct size (kb): 5.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 1.299999952316284
Tissue: liver polysomes
Gene product: cytochrome P450, 1a2, aromatic carbon inducible [P4503]
Alleles: a, b, a, b
Insert Size (kb) 1.300
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--4.4, 1.3; BamHI--5.6; HindIII--5.6; EcoRI--5.6; PstI--4.3, 1.0.
Murine P3-450 corresponds to rat P-450d and rabbit form 4.
The insert extends from the HindIII site at nt 579 through the poly(A) sequence.
Exhibits 29% nucleotide homology with rat P-450e; the protein homology is 22% with rat P-450e and 77% with rabbit form 2 protein.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Hildebrand CE, et al. Regional linkage analysis of the dioxin-inducible P-450 gene family on mouse chromosome 9. Biochem. Biophys. Res. Commun. 130: 396-406, 1985. PubMed: 4040754

Kimura S, et al. The murine Ah locus. Comparison of the complete cytochrome P1-450 and P3-450 cDNA nucleotide and amino acid sequences. J. Biol. Chem. 259: 10705-10713, 1984. PubMed: 6547952

Prochazka M, et al. Three recessive loci required for insulin-dependent diabetes in nonobese diabetic mice. Science 237: 286-289, 1987. PubMed: 2885918

Dey A, et al. Cell-specific induction of mouse Cyp1a1 mRNA during development. Proc. Natl. Acad. Sci. USA 86: 7446-7450, 1989. PubMed: 2477842

Daniel W Nebert, personal communication

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