26P (ATCC® 61808)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: J Mandel

Permits and Restrictions

View Permits

Designations 26P
Species Homo sapiens, human
Depositors J Mandel
Vector
Construct size (kb): 9.399999618530273
Insert
DNA: genomic
Insert lengths(kb): 5.599999904632568
Tissue: lymphoblast (49,XXXXY) cell line
Gene product: DNA Segment, single copy [D9S5]
Alleles: B1, B2, B3
Insert Size (kb) 5.600
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI/SalI--5.6, 3.8; AvaI--3.5, 3.2, 1.44, 0.82 + smaller; EcoRI--9.4; BglI--7.55, 1.85; HindIII--9.4.
The insert contains the following restriction sites (in approximate kb from the PstI site): NotI--0.44; BssHII--0.96.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
This probe is approximately 15 kb from DR47.
Detects genomic restriction fragments of the following sizes (complete digest, kb): NotI--450; EagI--260; BsshII--160; SacII--160.
The ClaI and the BamHI sites of the polylinker are absent as a result of the probe construction. All other sites of the polylinker are retained.
This was subcloned from the end of the cosmid c26 (Cos 26) by digestion with PstI and religation of the vector/insert construct at low DNA concentration.
References

Fujita R, et al. Additional polymorphisms at marker loci D9S5 and D9S15 generate extended haplotypes in linkage disequilibrium with Friedreich ataxia. Proc. Natl. Acad. Sci. USA 87: 1796-1800, 1990. PubMed: 1968638

Hanauer A, et al. The Friedreich ataxia gene is assigned to chromosoem 9q13-q21 by mapping of tightly linked markers and shows linkage disequilibrium with D9S15. Am. J. Hum. Genet. 46: 133-137, 1990. PubMed: 2294745

Fujita R, et al. Physical mapping of two loci (D9S5 and D9S15) tightly linked to Friedreich ataxia locus (FRDA) and identification of nearby CpG islands by pulse-field electrophoresis. Genomics 10: 915-920, 1991. PubMed: 1916823

Jean Louis Mandel, personal communication

Product Sheet
Product Sheet
Product Sheet