CRI-O136 (ATCC® 61780)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: Collaborative Research, Inc.

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Designations CRI-O136
Species Homo sapiens, human
Depositors Collaborative Research, Inc.
Construct size (kb): 40.40000152587891
DNA: genomic
Insert lengths(kb): 33.59999847412109
Gene product: DNA Segment, single copy [D16S60]
Alleles: A1, A2, A3, B1, B2
Insert Size (kb) 33.600
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Restriction digests of the clone give the following sizes (kb): PstI--9.0, 7.2, 4.3 (doublet), 4.1, 3.5, 2.3, 2.0; BglII--9.3 (doublet), 6.0, 5.6, 3.7, 3.2, 2.0, 1.9;
EcoRI--6.2, 5.6, 4.8, 3.5, 2.9 (doublet), 2.6 (doublet), 2.1, 2.0 (doublet), 1.7, 0.9; HindIII--16.0, 13.0, 9.0, 7.0; BamHI--23.3, 13.0, 2.2.
The insert can be excised from the vector with EcoRI, resulting in a 5.5 kb EcoRI vector fragment.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
The HincII polymorphisms have a combined PIC value of 0.81 and a combined heterozygosity of 83%.
Enzyme(s) not detecting polymorphism: BamHI, EcoRI, PstI, RsaI (panel of 5 individuals).

Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638

Keith TP, et al. Genetic linkage map of 43 DNA markers on human chromosome 16. Proc. Natl. Acad. Sci. USA 87: 5754-5758, 1990. PubMed: 2377614

Reeders ST, et al. Regional localisation of the autosomal dominant polycystic kidney disease locus. Genomics 3: 150-155, 1988. PubMed: 2906325

Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474

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