lambdaHGME (ATCC® 61531)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations lambdaHGME
GenBank Number

X16504

Species Homo sapiens, human
Vector
Construct size (kb): 46.5
Insert
DNA: genomic
Insert lengths(kb): 17.5
Tissue: lymphocyte
Gene product: enolase 3, (beta, muscle)(enolase, beta, muscle-specific) [ENO3]
Insert Size (kb) 17.5
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--uncut; SacI--20.0, 8.8, 4.4, 3.2, 2.1, 1.8, 1.3, 1.0 + smaller; XhoI--20.0, 8.8 (doublet), 7.4, 1.4; XbaI--20.0, 8.8, 7.4, 4.4, 2.1 + smaller; BglII--22.0, 7.0, 5.6, 4.3, 3.2, 1.5;
KpnI--23.0, 6.6, 5.2, 3.7, 3.1, 0.61 + smaller.
SacI, XbaI and XhoI sites flank the insert. An XhoI digest gives fragments of 8.8, 7.4, and 1.3 kb from the insert. The 8.8 kb fragment bears the entire gene.
References

Peshavaria M, et al. Structure of human muscle (beta) enolase mRNA and protein deduced from a genomic clone. Nucleic Acids Res. 17: 8862, 1989. PubMed: 2587223

Ian N Day, personal communication

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