F814 (ATCC® 61374)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations F814
Species Homo sapiens, human
Applications
This probe can be used routinely to detect both the F8C and DXS52 (VNTR) RFLPs on 0.9 - 1.0 agarose gels.
Vector
Construct size (kb): 4.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 1.5
Gene product: coagulation factor VIIIc, procoagulant component (hemophilia A) [F8C]
Alleles: F3, E1, E1, E2, F1, F2, B1, F1, F2, A1, A2, B2
Insert Size (kb) 1.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.2; EcoRI/HindIII--2.7, 1.5; HindIII--2.7, 1.5; PstI--3.5, 0.68; XbaI--4.2.
Linkage has been demonstrated between X-linked recessive hydrocephalus (HSAS) and DXS52 (theta = 0.03, LOD score = 6.52). Linkage has also been demonstrated between HSAS and F8 (theta = 0.00, LOD score = 4.32).
This is a hybrid probe containing a fragment of the factor VIII gene that detects the BclI RFLP and a fragment close to the St14 minisatellite that detects the VNTR in several restriction digests including TaqI, BclI, and MspI.
This probe can be used routinely to detect both the F8C and DXS52 (VNTR) RFLPs on 0.9 - 1.0 agarose gels. It also gives a clear pattern for DXS52 on TaqI blots.
This probe does not detect the 2-allele RFLPs of St14-1 (ATCC 61372) (TaqI, BclI).
References

Mandel JL, et al. Genetic mapping of the human X chromosome: linkage analysis of the q26-q28 region that includes the fragile X locus and isolation of expressed sequences. Cold Spring Harbor Symp. Quant. Biol. 51: 195-203, 1986. PubMed: 3472716

Heilig R, et al. Improved DNA markers for efficient analysis of fragile X families. Am. J. Med. Genet. 30: 543-550, 1988. PubMed: 2902795

Vincent A, et al. The polymorphic marker DXS304 is within 5 centimorgans of the fragile X locus. Genomics 5: 797-801, 1989. PubMed: 2574147

Willems PJ, et al. Assignment of X-linked hydrocephalus to Xq28 by linkage analysis. Genomics 8: 367-370, 1990. PubMed: 1979056

Jean Louis Mandel, personal communication

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