Y-223a (ATCC® 61170)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: U Muller

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Designations Y-223a
Species Homo sapiens, human
Depositors U Muller
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 0.75
Tissue: 49,XXXXY cell line
Gene product: DNA Segment, repetitive [DYZ5]
Insert Size (kb) 0.75
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.2; HindIII--4.4, 0.76; NotI--uncut; EcoRI--5.2; PstI--5.2.
Y-223a maps closer to the centromere than Y-280.
Y-223a hybridizes to DNA in 9/16 46,XX males and a female with a dicentric Y chromosome.
References

Muller U, et al. Deletion mapping of the testis determining locus with DNA probes in 46,XX males and in 46,XY and 46,X,dic(Y) females. Nucleic Acids Res. 14: 6489-6505, 1986. PubMed: 3748818

Gaunt SJ. Mapping of testis-determining locus on Yp by the molecular genetic analysis of XX males and XY females. Development 101: 51-58, 1987. PubMed: 88211484

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