Y-223a (ATCC® 61170)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: U Muller

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Designations Y-223a
Species Homo sapiens, human
Depositors U Muller
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 0.75
Tissue: 49,XXXXY cell line
Gene product: DNA Segment, repetitive [DYZ5]
Insert Size (kb) 0.75
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.2; HindIII--4.4, 0.76; NotI--uncut; EcoRI--5.2; PstI--5.2.
Y-223a maps closer to the centromere than Y-280.
Y-223a hybridizes to DNA in 9/16 46,XX males and a female with a dicentric Y chromosome.
References

Muller U, et al. Deletion mapping of the testis determining locus with DNA probes in 46,XX males and in 46,XY and 46,X,dic(Y) females. Nucleic Acids Res. 14: 6489-6505, 1986. PubMed: 3748818

Gaunt SJ. Mapping of testis-determining locus on Yp by the molecular genetic analysis of XX males and XY females. Development 101: 51-58, 1987. PubMed: 88211484

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