pHuR98 [pHuR 98] (ATCC® 61078)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: RK Moyzis

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Designations pHuR98 [pHuR 98]
GenBank Number

M20262

M20262

Species Homo sapiens, human
Depositors RK Moyzis
Vector
Construct size (kb): 4.599999904632568
Insert
DNA: genomic
Insert lengths(kb): 0.1580000072717667
Tissue: placenta
Gene product: DNA Segment, repetitive [D9Z3]
Insert Size (kb) 0.158
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.5; EcoRI--4.5; HindIII--4.5; NdeI--4.5; PstI--4.3, 0.21.
DNA containing this sequence is underrepresented in most genomic libraries because most restriction enzymes (except HinfI and TaqI) generate fragments with pHuR98 elements larger than 20 kb.
The repeat sequence of this member of the satellite 3 family consists of GAACCCGAGT followed by diverged GGAAT repeats. This insert contains repeat spacing of 54 and 45 nt, separated by a 29 nt repeat.
References

Moyzis RK, et al. Human chromosome-specific repetitive DNA sequences: novel markers for genetic analysis. Chromosoma 95: 375-386, 1987. PubMed: 3677921

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