pHuR98 [pHuR 98] (ATCC® 61078)

Clone Type: Clone  /  Depositors: RK Moyzis

Permits and Restrictions

View Permits

Designations pHuR98 [pHuR 98]
GenBank Number

M20262

Depositors RK Moyzis
Vector
Construct size (kb): 4.60
Name of vector: pBR322
Intact vector size: 4.36
Type of vector: plasmid
Vector end: PstI
Vector end: PstI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli

Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749
Insert
DNA: Genomic
Insert lengths (kb): 0.16
Tissue: Placenta
Gene product: DNA Segment, repetitive [D9Z3]
Genome: human
Gene symbol: D9Z3
Contains complete coding sequence?: U
Chromosome: 9; Localization: 9 q12
Insert end: Modification: GC tails
Cross references: DNA Seq. Acc.: M20262
Insert Size (kb) 0.158
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--4.5; EcoRI--4.5; HindIII--4.5; NdeI--4.5; PstI--4.3, 0.21.
DNA containing this sequence is underrepresented in most genomic libraries because most restriction enzymes (except HinfI and TaqI) generate fragments with pHuR98 elements larger than 20 kb.
The repeat sequence of this member of the satellite 3 family consists of GAACCCGAGT followed by diverged GGAAT repeats. This insert contains repeat spacing of 54 and 45 nt, separated by a 29 nt repeat.
References

Moyzis RK, et al. Human chromosome-specific repetitive DNA sequences: novel markers for genetic analysis. Chromosoma 95: 375-386, 1987. PubMed: 3677921