pHHH208 (ATCC® 59990)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: RL White, Y Nakamura

Permits and Restrictions

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Designations pHHH208
Species Homo sapiens, human
Depositors RL White, Y Nakamura
Applications
Codominant segregation of the BamHI RFLP was observed in 39 three-generation families.
Vector
Construct size (kb): 9.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 6.5
Gene product: DNA Segment, single copy [D14S19]
Alleles: A3, A3, A1, A1, A2, A2
Insert Size (kb) 6.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.5, 2.6; EcoRI--8.5, 0.58; HindIII/EcoRI--4.0, 2.6, 1.05, 0.87, 0.58; HindIII--7.7, 0.87, 1.05; SalI--9.5; consistent with deposited DNA.
Codominant segregation of the BamHI RFLP was observed in 39 three-generation families.
This was subcloned from cosmid HHH208.
Enzyme(s) not detecting polymorphism: MspI, TaqI, BglII, PstI, PvuII and RsaI.
References

Hoff M, et al. Isolation and mapping of a polymorphic DNA sequence (pHHH208) on chromosome 14 [D14S19]. Nucleic Acids Res. 16: 10400, 1988. PubMed: 2904135

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