pHHH208 (ATCC® 59990)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: RL White, Y Nakamura

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Designations pHHH208
Species Homo sapiens, human
Depositors RL White, Y Nakamura
Applications
Codominant segregation of the BamHI RFLP was observed in 39 three-generation families.
Vector
Construct size (kb): 9.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 6.5
Gene product: DNA Segment, single copy [D14S19]
Alleles: A3, A3, A1, A1, A2, A2
Insert Size (kb) 6.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.5, 2.6; EcoRI--8.5, 0.58; HindIII/EcoRI--4.0, 2.6, 1.05, 0.87, 0.58; HindIII--7.7, 0.87, 1.05; SalI--9.5; consistent with deposited DNA.
Codominant segregation of the BamHI RFLP was observed in 39 three-generation families.
This was subcloned from cosmid HHH208.
Enzyme(s) not detecting polymorphism: MspI, TaqI, BglII, PstI, PvuII and RsaI.
References

Hoff M, et al. Isolation and mapping of a polymorphic DNA sequence (pHHH208) on chromosome 14 [D14S19]. Nucleic Acids Res. 16: 10400, 1988. PubMed: 2904135

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