pCMM65 (ATCC® 59932)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: RL White, Y Nakamura

Permits and Restrictions

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Designations pCMM65
Species Homo sapiens, human
Depositors RL White, Y Nakamura
Applications
RFLPs are observed under normal hybridization and wash stringencies.
Vector
Construct size (kb): 7.099999904632568
Insert
DNA: genomic
Insert lengths(kb): 4.400000095367432
Gene product: DNA Segment, single copy [D16S84]
Alleles: A1, A1, A2, A2, A1, A2
Insert Size (kb) 4.400
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.0, 2.5; EcoRI--5.4; HindIII--5.4; BamHI--3.2, 2.3; PstI--2.5, 2.05, 0.46, 0.42, 0.22; consistent with deposited DNA.
RFLPs are observed under normal hybridization and wash stringencies.
References

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pCMM65) on chromosome 16 [D16S84]. Nucleic Acids Res. 16: 3122, 1988. PubMed: 2897115

Harris PC, et al. A long-range restriction map between the alpha-globin complex and a marker closely linked to the polycystic kidney disease 1 (PKD1) locus. Genomics 7: 195-206, 1990. PubMed: 2347584

Germino GG, et al. Identification of a locus which shows no genetic recombination with the autosomal dominant polycystic kidney disease gene on chromosome 16. Am. J. Hum. Genet. 46: 925-933, 1990. PubMed: 2339691

Julier C, et al. A primary map of 24 loci on human chromosome 16. Genomics 6: 419-427, 1990. PubMed: 2328986

Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872

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