pJM128 (ATCC® 59904)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: TA Donlon

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Designations pJM128
Species Homo sapiens, human
Depositors TA Donlon
Vector
Construct size (kb): 6.900000095367432
Insert
DNA: genomic
Insert lengths(kb): 2.549999952316284
Gene product: DNA Segment, repetitive [D8Z2]
Insert Size (kb) 2.550
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
The insert contains RsaI (5), PstI (1), and XbaI (2) sites.
Restriction digests of the clone give the following sizes (kb): HindIII--4.3, 2.5; PstI--4.0, 2.9; XbaI--6.3, 0.66; XbaI/HindIII--4.3, 1.4, 0.66; XbaI/PstI--4.0, 2.2, 0.66.
This is a subclone of lambda285-1225 (the central 2.55 kb HindIII fragment) and contains 15 repeat monomers.
References

Donlon T, et al. Alpha satellite-like sequences at the centromere of chromosome #8. Am. J. Hum. Genet. 39: A196, 1986.

Choo KH, et al. A survey of the genomic distribution of alpha satellite DNA on all the human chromosomes, and derivation of a new consensus sequence. Nucleic Acids Res. 19: 1179-1182, 1991. PubMed: 2030938

Timothy A Donlon, personal communication

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