ASSg1 (ATCC® 59877)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations ASSg1
Species Homo sapiens, human
Vector
Construct size (kb): 3.299999952316284
Insert
DNA: genomic
Insert lengths(kb): 0.449999988079071
Tissue: fetal liver
Gene product: argininosuccinate synthetase(arginosuccinate synthetase 1) [ASS]
Alleles: A1, A1, A1, A2, A2, A2
Insert Size (kb) 0.450
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/PvuII--2.6, 0.47, 0.24; EcoRI--3.3; HindIII--3.3; EcoRI/HindIII--2.85, 0.42; PvuII--2.55, 0.68.
All of the RFLPs detected are in complete linkage disequilibrium suggesting a deletion accounts for the variation.
Insert contains sequence from the 14th intron of the argininosuccinate synthetase gene.
HindIII RFLP is also detected by MboI, BglII, XbaI and SstI. The population studied was CEPH (Centre d'Etude du Polymorphisme Humain) families.
Enzyme(s) not detecting polymorphism: BclI, BanI, HindII, SphI, XmnI, StuI, BamHI, PstI, EcoRI, DdeI, RsaI, BglI, PvuII, TaqI.
References

Northrup H, et al. Multilocus linkage analysis with the human argininosuccinate synthetase gene. Genomics 5: 442-444, 1989. PubMed: 2575581

Hope Northrup, personal communication

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