cMCOD13 (ATCC® 59851)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations cMCOD13
Species Homo sapiens, human
Applications
RFLPs were observed after competitive hybridization with total human DNA.
Vector
Construct size (kb): 43.0
Insert
DNA: genomic
Insert lengths(kb): 35.0
Gene product: DNA Segment, single copy [D3S44]
Insert Size (kb) 35.0
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--16.5, 15.5, 10.0, 2.95, 2.85, 0.95, 0.92; EcoRI--15.5, 15.0, 14.5, 8.1; HindIII--23.0, 16.0, 3.65;
PstI--15.5, 7.1, 4.4, 3.85, 2.55, 2.4, 2.0, 1.3, 1.2, 0.95, 0.76, 0.73, 0.52; EcoRI/HindIII--15.0, 13.0, 12.5, 4.1, 3.65, 1.9, 1.75, 0.65; consistent with deposited DNA.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
RFLPs were observed after competitive hybridization with total human DNA.
Enzyme(s) not detecting polymorphism: BglII.
References

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (cMCOD13) on chromosome 3 (D3S44). Nucleic Acids Res. 16: 9356, 1988. PubMed: 2902583

Nakamura Y, et al. New approach for isolation of VNTR markers. Am. J. Hum. Genet. 43: 854-859, 1988. PubMed: 2904220

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