cMCOD13 (ATCC® 59851)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

View Permits

Designations cMCOD13
Species Homo sapiens, human
Applications
RFLPs were observed after competitive hybridization with total human DNA.
Vector
Construct size (kb): 43.0
Insert
DNA: genomic
Insert lengths(kb): 35.0
Gene product: DNA Segment, single copy [D3S44]
Insert Size (kb) 35.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--16.5, 15.5, 10.0, 2.95, 2.85, 0.95, 0.92; EcoRI--15.5, 15.0, 14.5, 8.1; HindIII--23.0, 16.0, 3.65;
PstI--15.5, 7.1, 4.4, 3.85, 2.55, 2.4, 2.0, 1.3, 1.2, 0.95, 0.76, 0.73, 0.52; EcoRI/HindIII--15.0, 13.0, 12.5, 4.1, 3.65, 1.9, 1.75, 0.65; consistent with deposited DNA.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
RFLPs were observed after competitive hybridization with total human DNA.
Enzyme(s) not detecting polymorphism: BglII.
References

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (cMCOD13) on chromosome 3 (D3S44). Nucleic Acids Res. 16: 9356, 1988. PubMed: 2902583

Nakamura Y, et al. New approach for isolation of VNTR markers. Am. J. Hum. Genet. 43: 854-859, 1988. PubMed: 2904220

Product Sheet
Product Sheet
Product Sheet