pMCK2 (ATCC® 59821)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pMCK2
Species Homo sapiens, human
Applications
Codominant segregation of the PvuII RFLP was observed in 48 three-generation families.
Vector
Construct size (kb): 4.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 2.0
Gene product: DNA Segment, single copy [D10S15]
Insert Size (kb) 2.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.0; HindIII--5.0; BamHI--5.0; PstI--2.8, 1.5, 0.5; EcoRI--2.8, 2.15, consistent with deposited DNA.
pMCT50 (ATCC 57660, 57661) is the same as pMCK2 (ATCC 59820, 59821).
Codominant segregation of the PvuII RFLP was observed in 48 three-generation families.
Enzyme(s) not detecting polymorphism: TaqI and BglII.
References

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pMCK2 on chromosome 10 (D10S15). Nucleic Acids Res. 16: 374, 1988. PubMed: 2448751

Wu JS, et al. The genetic defect in multiple endocrine neoplasia type 2A maps next to the centromere of chromosome 10. Am. J. Hum. Genet. 46: 624-630, 1990. PubMed: 1968709

White RL, et al. The CEPH consortium primary linkage map of human chromosome 10. Genomics 6: 393-412, 1990. PubMed: 1970325

Raymond L White, personal communication

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