pMCK2 (ATCC® 59821)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pMCK2
Species Homo sapiens, human
Applications
Codominant segregation of the PvuII RFLP was observed in 48 three-generation families.
Vector
Construct size (kb): 4.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 2.0
Gene product: DNA Segment, single copy [D10S15]
Insert Size (kb) 2.0
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.0; HindIII--5.0; BamHI--5.0; PstI--2.8, 1.5, 0.5; EcoRI--2.8, 2.15, consistent with deposited DNA.
pMCT50 (ATCC 57660, 57661) is the same as pMCK2 (ATCC 59820, 59821).
Codominant segregation of the PvuII RFLP was observed in 48 three-generation families.
Enzyme(s) not detecting polymorphism: TaqI and BglII.
References

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pMCK2 on chromosome 10 (D10S15). Nucleic Acids Res. 16: 374, 1988. PubMed: 2448751

Wu JS, et al. The genetic defect in multiple endocrine neoplasia type 2A maps next to the centromere of chromosome 10. Am. J. Hum. Genet. 46: 624-630, 1990. PubMed: 1968709

White RL, et al. The CEPH consortium primary linkage map of human chromosome 10. Genomics 6: 393-412, 1990. PubMed: 1970325

Raymond L White, personal communication

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