pML34 [p34] (ATCC® 59715)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pML34 [p34]
Species Homo sapiens, human
Vector
Construct size (kb): 10.60000038146973
Insert
DNA: genomic
Insert lengths(kb): 6.199999809265137
Gene product: zinc finger protein 127(zinc finger protein ZNF127) [D15S9]
Alleles: A1, A2, B1, B2, A1, A2, B1, B2
Insert Size (kb) 6.200
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 6.2; BamHI--6.8, 3.8; EcoRI--10.1, 0.5; SalI--10.6; EcoRI/SalI--6.4, 3.9, 0.5.
Insert contains the following sites separated by (kb): HindIII- 0.5- EcoRI- 2.2- BamHI- 1.0- ScaI- 2.5- HindIII.
Enzyme(s) not detecting polymorphism: DraI, NciI, Eco0109I (8 unrelated individuals).
References

Donlon TA, et al. Isolation of molecular probes associated with the chromosome 15 instability in the Prader-Willi syndrome. Proc. Natl. Acad. Sci. USA 83: 4408-4412, 1986. PubMed: 3012567

Nicholls RD, et al. Restriction fragment length polymorphisms with proximal 15q and their use in molecular cytogenetics and the Prader-Willi syndrome. Am. J. Med. Genet. 33: 66-77, 1989. PubMed: 2568752

Hamabe J, et al. A BsaBI RFLP detected for probe pML34 [D15S9] on chromosome 15q. Nucleic Acids Res. 19: 4789, 1991. PubMed: 1679924

Marc Lalande, personal communication

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