pML34 [p34] (ATCC® 59715)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pML34 [p34]
Species Homo sapiens, human
Vector
Construct size (kb): 10.60000038146973
Insert
DNA: genomic
Insert lengths(kb): 6.199999809265137
Gene product: zinc finger protein 127(zinc finger protein ZNF127) [D15S9]
Alleles: A1, A2, B1, B2, A1, A2, B1, B2
Insert Size (kb) 6.200
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 6.2; BamHI--6.8, 3.8; EcoRI--10.1, 0.5; SalI--10.6; EcoRI/SalI--6.4, 3.9, 0.5.
Insert contains the following sites separated by (kb): HindIII- 0.5- EcoRI- 2.2- BamHI- 1.0- ScaI- 2.5- HindIII.
Enzyme(s) not detecting polymorphism: DraI, NciI, Eco0109I (8 unrelated individuals).
References

Donlon TA, et al. Isolation of molecular probes associated with the chromosome 15 instability in the Prader-Willi syndrome. Proc. Natl. Acad. Sci. USA 83: 4408-4412, 1986. PubMed: 3012567

Nicholls RD, et al. Restriction fragment length polymorphisms with proximal 15q and their use in molecular cytogenetics and the Prader-Willi syndrome. Am. J. Med. Genet. 33: 66-77, 1989. PubMed: 2568752

Hamabe J, et al. A BsaBI RFLP detected for probe pML34 [D15S9] on chromosome 15q. Nucleic Acids Res. 19: 4789, 1991. PubMed: 1679924

Marc Lalande, personal communication

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