pJlambda2 (ATCC® 59616)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: M Lefranc

Permits and Restrictions

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Designations pJlambda2
Species Homo sapiens, human
Depositors M Lefranc
Vector
Construct size (kb): 4.900000095367432
Insert
DNA: genomic
Insert lengths(kb): 2.0
Gene product: immunoglobulin lambda joining segment J2 [IGLJ2*]
Alleles: A1, A2, A3, A4
Insert Size (kb) 2.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BglII/EcoRI--4.4; BglII-uncut; EcoRI--4.9; PstI--2.75, 1.05, 0.80; HindIII--4.9; EcoRI/PstI--2.7, 1.25, 1.0; SalI--5.4.
A 2.0 kb BglII/EcoRI fragment containing the Jlambda2 segment (IGLJ2).
References

Dariavach P, et al. Human immunoglobulin Clambda6 gene encodes the Kern+Oz- lambda chain and Clambda4 and Clambda5 are pseudogenes. Proc. Natl. Acad. Sci. USA 84: 9074-9078, 1987. PubMed: 3122211

Ghanem N, et al. Polymorphism of immunoglobulin lambda constant region genes in populations from France, Lebanon and Tunisia. Exp. Clin. Immunogenet. 5: 186-195, 1988. PubMed: 2908491

Product Sheet
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Product Sheet