pJlambda2 (ATCC® 59616)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: M Lefranc

Designations pJlambda2
Species Homo sapiens, human
Depositors M Lefranc
Vector
Construct size (kb): 4.900000095367432
Insert
DNA: genomic
Insert lengths(kb): 2.0
Gene product: immunoglobulin lambda joining segment J2 [IGLJ2*]
Alleles: A1, A2, A3, A4
Insert Size (kb) 2.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BglII/EcoRI--4.4; BglII-uncut; EcoRI--4.9; PstI--2.75, 1.05, 0.80; HindIII--4.9; EcoRI/PstI--2.7, 1.25, 1.0; SalI--5.4.
A 2.0 kb BglII/EcoRI fragment containing the Jlambda2 segment (IGLJ2).
References

Dariavach P, et al. Human immunoglobulin Clambda6 gene encodes the Kern+Oz- lambda chain and Clambda4 and Clambda5 are pseudogenes. Proc. Natl. Acad. Sci. USA 84: 9074-9078, 1987. PubMed: 3122211

Ghanem N, et al. Polymorphism of immunoglobulin lambda constant region genes in populations from France, Lebanon and Tunisia. Exp. Clin. Immunogenet. 5: 186-195, 1988. PubMed: 2908491

Product Sheet
Product Sheet
Product Sheet