pCMM66 (ATCC® 59371)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pCMM66
Species Homo sapiens, human
Applications
A 1.8 kb EcoRI/HindIII fragment has been used to detect the PstI polymorphism.
Otherwise, RFLPs were observed under normal hybridization and wash stringencies.
Vector
Construct size (kb): 7.099999904632568
Insert
DNA: genomic
Insert lengths(kb): 4.800000190734863
Gene product: DNA Segment, single copy [D14S22]
Insert Size (kb) 4.800
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.55, 1.75, 1.55, 1.2; EcoRI--5.2, 1.65; AccI--4.1, 2.7, consistent with deposited DNA.
Codominant segregation demonstrated in 34 3-generation families for the PstI RFLP.
A 1.8 kb EcoRI/HindIII fragment has been used to detect the PstI polymorphism.
The probe was pre-associated with excess human DNA before hybridization. Otherwise, RFLPs were observed under normal hybridization and wash stringencies.
Enzyme(s) not detecting polymorphism: MspI, TaqI.
References

Wang JC, et al. Uniparental heterodisomy for chromosome 14 in a phenotypically abnormal familial balanced 13/14 Robertsonian translocation carrier. Am. J. Hum. Genet. 48: 1069-1074, 1991. PubMed: 2035528

Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pCMM66) on chromosome 14 [D14S22]. Nucleic Acids Res. 16: 6255, 1988. PubMed: 2899877

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