phpim5R1 [hpim-1, pHPimR1] (ATCC® 59169)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations phpim5R1 [hpim-1, pHPimR1]
Species Homo sapiens, human
Vector
Construct size (kb): 6.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 3.599999904632568
Tissue: 380 cell line
Gene product: proviral integration, MCF [PIM]
Alleles: A1, A2, B1, B2, C1, A1, A2, B1, B2, C1, C2, C2
Insert Size (kb) 3.600
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 3.05; HindIII--3.6, 2.5, 0.9; PstI--2.45, 1.5, 1.2, 1.1, 0.54; BamHI--5.2, 1.2, 0.48; SalI--7.0.
Insert contains BamHI (2) and HindIII (2) sites.
Includes part of intron 4, exon 5, intron 5, exon 6, and 3' flanking sequences.
Cross-hybridizes to mouse Pim-1 gene under stringent conditions.
Vector PyT is a pBR322 derivative with a deletion between EcoRII sites 1058 and 2500.
Enzyme(s) not detecting polymorphism: EcoRI, HindIII.
References

Zoghbi HY, et al. Deletion and linkage mapping of eight markers from the proximal short arm of chromosome 6. Genomics 6: 352-357, 1990. PubMed: 1968423

Nagarajan L, et al. Localization of the human pim oncogene (PIM) to a region of chromosome 6 involved in translocations in acute leukemias. Proc. Natl. Acad. Sci. USA 83: 2556-2560, 1986. PubMed: 3458216

Meeker TC, et al. Characterization of the human PIM-1 gene: a putative proto-oncogene coding for a tissue specific member of the protein kinase family. Oncogene Res. 1: 87-101, 1987. PubMed: 3329711

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