TL11 (ATCC® 57688)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: L Warnich

Permits and Restrictions

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Designations TL11
Species Homo sapiens, human
Depositors L Warnich
Applications
Prehybridization with human DNA is recommended.
Enzyme(s) not detecting polymorphism: EcoRI, BamHI, MspI, TaqI, PstI (tested against 8 unrelated Caucasians).
Vector
Construct size (kb): 4.300000190734863
Insert
DNA: genomic
Insert lengths(kb): 9.300000190734863
Gene product: DNA Segment, single copy [D8S5]
Alleles: B1, B2, A1, A2
Insert Size (kb) 9.300
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--22, 8 (arms), 9.4 (insert); EcoRI--22, 7.5, 6.2, 4.9, 2.4; BamHI/EcoRI--22, 7.5, 4.9, 4.3, 2.2, 1.8.
The map location has been revised to 8p23-q11.
Insert contains EcoRI (4.3 kb, 9.2 kb) sites (distance in kb from 23.58 arm).
Prehybridization with human DNA is recommended.
Codominant segregation of the HindIII polymorphism has been demonstrated in 6 families (29 individuals).
Enzyme(s) not detecting polymorphism: EcoRI, BamHI, MspI, TaqI, PstI (tested against 8 unrelated Caucasians).
Enzyme(s) not detecting polymorphism: ApaI, BclI, BglI, BglII, DraI, HgiAI, MboI, NsiI, RsaI, SacI, XhoI.
References

Cottingham AW, et al. A new StyI RFLP and haplotypes with the HindIII RFLP at the D8S5 (TL11) locus. Nucleic Acids Res. 19: 4790, 1991. PubMed: 1679925

Dietzsch E, et al. An anonymous human single copy genomic clone (D8S5) (TL11) on chromosome 8 identifies a moderately frequent RFLP. Nucleic Acids Res. 14: 6781, 1986. PubMed: 3018683

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