TL11 (ATCC® 57688)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: L Warnich

Permits and Restrictions

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Designations TL11
Species Homo sapiens, human
Depositors L Warnich
Applications
Prehybridization with human DNA is recommended.
Enzyme(s) not detecting polymorphism: EcoRI, BamHI, MspI, TaqI, PstI (tested against 8 unrelated Caucasians).
Vector
Construct size (kb): 4.300000190734863
Insert
DNA: genomic
Insert lengths(kb): 9.300000190734863
Gene product: DNA Segment, single copy [D8S5]
Alleles: B1, B2, A1, A2
Insert Size (kb) 9.300
Biosafety Level 1
Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--22, 8 (arms), 9.4 (insert); EcoRI--22, 7.5, 6.2, 4.9, 2.4; BamHI/EcoRI--22, 7.5, 4.9, 4.3, 2.2, 1.8.
The map location has been revised to 8p23-q11.
Insert contains EcoRI (4.3 kb, 9.2 kb) sites (distance in kb from 23.58 arm).
Prehybridization with human DNA is recommended.
Codominant segregation of the HindIII polymorphism has been demonstrated in 6 families (29 individuals).
Enzyme(s) not detecting polymorphism: EcoRI, BamHI, MspI, TaqI, PstI (tested against 8 unrelated Caucasians).
Enzyme(s) not detecting polymorphism: ApaI, BclI, BglI, BglII, DraI, HgiAI, MboI, NsiI, RsaI, SacI, XhoI.
References

Cottingham AW, et al. A new StyI RFLP and haplotypes with the HindIII RFLP at the D8S5 (TL11) locus. Nucleic Acids Res. 19: 4790, 1991. PubMed: 1679925

Dietzsch E, et al. An anonymous human single copy genomic clone (D8S5) (TL11) on chromosome 8 identifies a moderately frequent RFLP. Nucleic Acids Res. 14: 6781, 1986. PubMed: 3018683

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