pH2.3 (ATCC® 57495)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pH2.3
GenBank Number

M11717

Species Homo sapiens, human
Vector
Construct size (kb): 5.699999809265137
Insert
DNA: genomic
Insert lengths(kb): 2.299999952316284
Gene product: heat shock 70 kDa protein(heat shock protein, 70 kDa) [HSPA*]
Alleles: A1, A2, , A1, A1, A2, A2, A2, A1
Insert Size (kb) 2.300
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): HindIII/BamHI--3.3, 2.3; HindIII--5.7; EcoRI--5.7; PvuII--uncut; PstI--3.5, 2.2.
This probe has been shown to contain the entire coding region for a heat-shock protein of 70 kDa and hybridizes to a polyadenlyated message of 2.6 kb.
Includes approximately 60 bp 5' of initiation ATG through approximately 280 bp 3' of termination TAG (including AATAAA). Genomic sequence is transcribed as an uninterrupted transcript of 2.44 kb with an ORF of 1.986 kb.
Enzyme(s) not detecting polymorphism (in a panel of 5): HincII, TaqI, MspI.
References

Zlotogora J, et al. Krabbe disease locus mapped to chromosome 14 by genetic linkage. Am. J. Hum. Genet. 47: 37-44, 1990. PubMed: 1971996

Hunt C, Morimoto RI. Conserved features of eukaryotic hsp70 genes revealed by comparison with the nucleotide sequence of human hsp70. Proc. Natl. Acad. Sci. USA 82: 6455-6459, 1985. PubMed: 3931075

Wu B, et al. Structure and expression of the human gene encoding major heat shock protein HSP70. Mol. Cell. Biol. 5: 330-341, 1985. PubMed: 2858050

Harrison GS, et al. Chromosomal location of human genes encoding major heat-shock protein HSP70. Somatic Cell Mol. Genet. 13: 119-130, 1987. PubMed: 3470951

Barbara Weiffenbach, personal communication

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