L1.4 [DOLUH1] (ATCC® 57296)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: E Bakker

Permits and Restrictions

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Designations L1.4 [DOLUH1]
Species Homo sapiens, human
Depositors E Bakker
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 0.7200000286102295
Gene product: DNA Segment, single copy [D5S4]
Alleles: B2, A1, A2, B1, A1, A2
Insert Size (kb) 0.720
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 0.75; BamHI--4.4; PstI--3.5, 0.94; AccI--4.4.
The RsaI polymorphism is in disequilibrium with the EcoRI RFLP.
Enzyme(s) not detecting polymorphism: BglII, HindIII, PvuII.
References

Hofker MH, et al. The X-chromosome shows less genetic variation at restriciton sites than the autosomes. Am. J. Hum. Genet. 39: 438-451, 1986. PubMed: 2876629

Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217

Weiffenbach B, et al. A genetic linkage map of human chromosome 5 with 60 RFLP loci. Genomics 10: 173-185, 1991. PubMed: 1675192

Derom C, et al. Zygosity determination in newborn twins using DNA variants. J. Med. Genet. 22: 279-282, 1985. PubMed: 2995674

Bailey CJ, et al. A new RFLP for l1.4 (D5S4) an anonymous genomic clone localised to chromosome 5. Nucleic Acids Res. 15: 6762, 1987. PubMed: 2888088

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