L1.4 [DOLUH1] (ATCC® 57296)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: E Bakker

Permits and Restrictions

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Designations L1.4 [DOLUH1]
Species Homo sapiens, human
Depositors E Bakker
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 0.7200000286102295
Gene product: DNA Segment, single copy [D5S4]
Alleles: B2, A1, A2, B1, A1, A2
Insert Size (kb) 0.720
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 0.75; BamHI--4.4; PstI--3.5, 0.94; AccI--4.4.
The RsaI polymorphism is in disequilibrium with the EcoRI RFLP.
Enzyme(s) not detecting polymorphism: BglII, HindIII, PvuII.
References

Hofker MH, et al. The X-chromosome shows less genetic variation at restriciton sites than the autosomes. Am. J. Hum. Genet. 39: 438-451, 1986. PubMed: 2876629

Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217

Weiffenbach B, et al. A genetic linkage map of human chromosome 5 with 60 RFLP loci. Genomics 10: 173-185, 1991. PubMed: 1675192

Derom C, et al. Zygosity determination in newborn twins using DNA variants. J. Med. Genet. 22: 279-282, 1985. PubMed: 2995674

Bailey CJ, et al. A new RFLP for l1.4 (D5S4) an anonymous genomic clone localised to chromosome 5. Nucleic Acids Res. 15: 6762, 1987. PubMed: 2888088

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