754 [L754] (ATCC® 57286)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: MH Hofker

Permits and Restrictions

View Permits

Designations 754 [L754]
Species Homo sapiens, human
Depositors MH Hofker
Vector
Construct size (kb): 5.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 2.200000047683716
Gene product: DNA Segment, single copy [DXS84]
Alleles: A2, A1
Insert Size (kb) 2.200
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--3.0, 2.2; BglI/PstI--4.0, 1.4; BglI/EcoRI--4.0, 1.4; PvuI/EcoRI--3.7, 1.6; PvuI/BamHI--3.0, 1.6, 0.9.
Shows linkage with X-linked retinitis pigmentosa (theta = 0.05, LOD score = 4.08).
The insert was isolated from a phage library derived from flow-sorted X chromosomes.
References

Musarella MA, et al. Localization of the gene for X-linked recessive type of retinitis pigmentosa (XLRP) to Xp21 by linkage analysis. Am. J. Hum. Genet. 43: 484-494, 1988. PubMed: 2902787

Hofker MH, et al. The X-chromosome shows less genetic variation at restriciton sites than the autosomes. Am. J. Hum. Genet. 39: 438-451, 1986. PubMed: 2876629

Bakker E, et al. Prenatal diagnosis and carrier detection of Duchenne muscular dystrophy with closely linked RFLPs. Lancet 1: 655-658, 1985. PubMed: 2858615

Hofker MH, et al. Development of additional RFLP probes near the locus for Duchenne muscular dystrophy by cosmid cloning of the DXS84 locus. Hum. Genet. 74: 270-274, 1986. PubMed: 2877935

Schwartz CE, et al. Allan-Herndon syndrome. II. Linkage to DNA markers in Xq21. Am. J. Hum. Genet. 47: 454-458, 1990. PubMed: 2393020

Thakker RV, et al. Linkage analysis of two cloned DNA sequences, DXS197 and DXS207, in hypophosphatemic rickets families. Genomics 8: 189-193, 1990. PubMed: 1979046

van Ommen GJ, et al. A physical map of 4 million bp around the Duchenne muscular dystrophy gene on the human X-chromosome. Cell 47: 499-504, 1986. PubMed: 2877741

Francke U, et al. Congenital adrenal hypoplasia, myopathy, and glycerol kinase deficiency: Molecular genetic evidence for deletions. Am. J. Hum. Genet. 40: 212-227, 1987. PubMed: 2883886

Hofker MH, et al. Isolation of probes detecting restriction fragment length polymorphisms from X chromosome-specific libraries: Potential use for diagnosis of Duchenne muscular dystrophy. Hum. Genet. 70: 148-156, 1985. PubMed: 2989153

Product Sheet
Product Sheet
Product Sheet