p43-15 (ATCC® 57163)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

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Designations p43-15
Species Homo sapiens, human
Vector
Construct size (kb): 8.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 3.799999952316284
Tissue: lymphoblast (49,XXXXY) cell line
Gene product: DNA Segment, single copy [DXS42]
Alleles: A2, A1, C1, C2
Insert Size (kb) 3.800
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
A unique sequence 1.8 kb fragment is generated by PstI/HindIII double digest.
Restriction digests of the clone give the following sizes (kb): BamHI--8.2; PstI--5.1, 1.8, 0.7, 0.5; BglII--8.2; HindIII--4.4, 3.7; HindIII/BglII--4.4, 3.5; HindIII/PstI--3.6, 1.6, 1.0, 0.78, 0.5.
Linked to the Lowe oculocerebrorenal syndrome (OCRL) (theta = 0, LOD score = 6.63).
References

Aldridge J, et al. A strategy to reveal high-frequency RFLPs along the human X chromosome. Am. J. Hum. Genet. 36: 546-564, 1984. PubMed: 6328976

Wadelius C, et al. Lowe oculocerebrorenal syndrome: DNA-based linkage of the gene to Xq24-q26, using tightly linked flanking markers and the correlation to lens examination in carrier diagnosis. Am. J. Hum. Genet. 44: 241-247, 1989. PubMed: 2912070

Reilly DS, et al. Genetic and physical mapping of Xq24-q26 markers flanking the Lowe oculocerebrorenal syndrome. Genomics 8: 62-70, 1990. PubMed: 2081601

Skare JC, et al. Mapping the X-linked lymphoproliferative syndrome. Proc. Natl. Acad. Sci. USA 84: 2015-2018, 1987. PubMed: 2882515

Reilly DS, et al. Tightly linked flanking markers for the Lowe oculocerebrorenal syndrome, with application to carrier assessment. Am. J. Hum. Genet. 42: 748-755, 1988. PubMed: 2895982

Lesko JG, Nussbaum RL. RFLP locus DXS42 is proximal to the locus for hypoxanthine phosphoribosyltransferase. Am. J. Hum. Genet. 39: 669-670, 1986. PubMed: 2878611

Louis M Kunkel, personal communication

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