pablK2 [ablK-2, pK2] (ATCC® 57102)

Organism: Murine leukemia virus, Murine leukemia virus  /  Clone Type: Clone  /  Depositors: RL White

Permits and Restrictions

View Permits

Designations pablK2 [ablK-2, pK2]
GenBank Number

JO2009

Species Murine leukemia virus, Murine leukemia virus
Depositors RL White
Vector
Construct size (kb): 5.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 1.5
Gene product: Abelson murine leukemia viral (v-abl) oncogene homolog 1 [ABL]
Alleles: D1, D2, D1, D2, A1, A2, B2, C1, C2, A1, A2, B1, B2, C1, C2, B1
Insert Size (kb) 1.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 1.4; BglI--2.3, 1.5, 1.2, 0.5, 0.2; SalI--4.2, 1.6; AvaI--4.0, 1.85; BamHI--5.8.
Previously incorrectly recorded as containing the human sequence. This is the same as ATCC 41034.
Corresponds to nt 2466 to nt 3912 on the A-MuLV map. The insert contains the following restriction sites (nt position according to published sequence): BglI--2815, 3396; AccI--2833; SstI--2842; PstI--3000, 3035, 3184; SalI--3517; HincII--3519.
Contains the p102 coding region and about 90 bp of 3' untranslated sequence.
The BclI polymorphism is uncharacterized.
Enzyme(s) not detecting polymorphism: MspI, EcoRI, BamHI, BglII, SstI, DdeI.
Classification Retroviridae,Gammaretrovirus,Mammalian virus group
References

Srinivasan A, et al. Abelson murine leukemia virus: molecular cloning of infectious integrated proviral DNA. Proc. Natl. Acad. Sci. USA 78: 2077-2081, 1981. PubMed: 6264461

Reddy EP, et al. Nucleotide sequence of Abelson murine leukemia virus genome: structural similarity of its transforming gene product to other onc gene products with tyrosine-specific kinase activity. Proc. Natl. Acad. Sci. USA 80: 3623-3627, 1983. PubMed: 6304726

Product Sheet
Product Sheet
Product Sheet