pablK2 [ablK-2, pK2] (ATCC® 57102)

Organism: Murine leukemia virus, Murine leukemia virus  /  Clone Type: Clone  /  Depositors: RL White

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Designations pablK2 [ablK-2, pK2]
GenBank Number

JO2009

Species Murine leukemia virus, Murine leukemia virus
Depositors RL White
Vector
Construct size (kb): 5.800000190734863
Insert
DNA: genomic
Insert lengths(kb): 1.5
Gene product: Abelson murine leukemia viral (v-abl) oncogene homolog 1 [ABL]
Alleles: D1, D2, D1, D2, A1, A2, B2, C1, C2, A1, A2, B1, B2, C1, C2, B1
Insert Size (kb) 1.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 1.4; BglI--2.3, 1.5, 1.2, 0.5, 0.2; SalI--4.2, 1.6; AvaI--4.0, 1.85; BamHI--5.8.
Previously incorrectly recorded as containing the human sequence. This is the same as ATCC 41034.
Corresponds to nt 2466 to nt 3912 on the A-MuLV map. The insert contains the following restriction sites (nt position according to published sequence): BglI--2815, 3396; AccI--2833; SstI--2842; PstI--3000, 3035, 3184; SalI--3517; HincII--3519.
Contains the p102 coding region and about 90 bp of 3' untranslated sequence.
The BclI polymorphism is uncharacterized.
Enzyme(s) not detecting polymorphism: MspI, EcoRI, BamHI, BglII, SstI, DdeI.
Classification Retroviridae,Gammaretrovirus,Mammalian virus group
References

Srinivasan A, et al. Abelson murine leukemia virus: molecular cloning of infectious integrated proviral DNA. Proc. Natl. Acad. Sci. USA 78: 2077-2081, 1981. PubMed: 6264461

Reddy EP, et al. Nucleotide sequence of Abelson murine leukemia virus genome: structural similarity of its transforming gene product to other onc gene products with tyrosine-specific kinase activity. Proc. Natl. Acad. Sci. USA 80: 3623-3627, 1983. PubMed: 6304726

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