pADJ762 (ATCC® 57100)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: RL White

Permits and Restrictions

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Designations pADJ762
Species Homo sapiens, human
Depositors RL White
Applications
A 0.9 kb EcoRI/SacI subfragment of the insert has also been used to detect the SacI polymorphism.
Vector
Construct size (kb): 11.5
Insert
DNA: genomic
Insert lengths(kb): 5.5
Gene product: DNA Segment, single copy [D11S12]
Alleles: A1, A2, B1, B2, C1, C2, D2, E1, E2, D1
Insert Size (kb) 5.5
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.0, 5.5; BamHI--5.2, 4.7, 2.1; PstI--6.2, 2.2, 1.4, 0.65, 0.54, 0.42, 0.34; AvaI--5.1, 4.2, 2.9; PvuII--5.1, 3.0, 2.15, 1.5, 0.6, 0.3.
A 0.9 kb EcoRI/SacI subfragment of the insert has also been used to detect the SacI polymorphism.
Enzyme(s) not detecting polymorphism: MboI, EcoRI, HindIII, BamHI, KpnI, PvuII, HincII, PstI, SstI, XmaI, HaeIII, BstNI, BstEII, AccI, StuI, XbaI, DdeI, HpaII.
References

Barker D, et al. A locus on chromosome 11p with multiple restriction site polymorphisms. Am. J. Hum. Genet. 36: 1159-1171, 1984. PubMed: 6097109

Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217

Beru N, Payton MN. BglII polymorphism at the human erythropoietin gene. Nucleic Acids Res. 19: 1717, 1991. PubMed: 1674133

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