pADJ762 (ATCC® 57100)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: RL White

Permits and Restrictions

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Designations pADJ762
Species Homo sapiens, human
Depositors RL White
Applications
A 0.9 kb EcoRI/SacI subfragment of the insert has also been used to detect the SacI polymorphism.
Vector
Construct size (kb): 11.5
Insert
DNA: genomic
Insert lengths(kb): 5.5
Gene product: DNA Segment, single copy [D11S12]
Alleles: A1, A2, B1, B2, C1, C2, D2, E1, E2, D1
Insert Size (kb) 5.5
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.0, 5.5; BamHI--5.2, 4.7, 2.1; PstI--6.2, 2.2, 1.4, 0.65, 0.54, 0.42, 0.34; AvaI--5.1, 4.2, 2.9; PvuII--5.1, 3.0, 2.15, 1.5, 0.6, 0.3.
A 0.9 kb EcoRI/SacI subfragment of the insert has also been used to detect the SacI polymorphism.
Enzyme(s) not detecting polymorphism: MboI, EcoRI, HindIII, BamHI, KpnI, PvuII, HincII, PstI, SstI, XmaI, HaeIII, BstNI, BstEII, AccI, StuI, XbaI, DdeI, HpaII.
References

Barker D, et al. A locus on chromosome 11p with multiple restriction site polymorphisms. Am. J. Hum. Genet. 36: 1159-1171, 1984. PubMed: 6097109

Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217

Beru N, Payton MN. BglII polymorphism at the human erythropoietin gene. Nucleic Acids Res. 19: 1717, 1991. PubMed: 1674133

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