pHcGAP (ATCC® 57091)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations pHcGAP
GenBank Number

M17851

Species Homo sapiens, human
Applications
standard for analysis of gene expression glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde 3-phosphate dehydrogenase, phosphoglyceraldehyde dehydrogenase, triosephosphate dehydrogenase
Vector
Construct size (kb): 5.599999904632568
Insert
DNA: cDNA
Insert lengths(kb): 1.200000047683716
Tissue: fetal liver
Gene product: glyceraldehyde-3-phosphate dehydrogenase(glyceraldehyde 3-phosphate dehydrogenase, phosphoglyceraldehyde dehydrogenase, triosephosphate dehydrogenase) [GAPD]
Target Gene: glyceraldehyde-3-phosphate dehydrogenase
Insert Size (kb) 1.200
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
The PstI site at the 3' end of the insert was destroyed during cloning. The retained PstI site is 750 bp from the EcoRI site. The insert contains a HindIII and a XbaI site.
Restriction digests of the clone give the following sizes (kb): PstI--5.55; HindIII--4.5, 1.06; RsaI--2.1, 1.4, 0.86, 0.65, 0.56; XbaI/PstI--4.7, 0.8; BglI--2.9, 2.2, 0.4, 0.2; BglI/PstI--2.2, 1.8, 1.0, 0.4, 0.2.
Includes 31 bp of 5' and 216 bp of 3' untranslated sequence, and a complete 1008 bp coding sequence. The polyadenylation signal AATAA is 15 bp upstream from poly(A) site.
The XbaI site is about 780 bp from the 5' PstI site. A PstI/XbaI double digest should give a probe containing the 5' non-coding sequences and sequences encoding about the first 250 amino acids.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
Thirty hybridizing bands are detected in genomic blots at 37C, 50% formamide, 5X SSC; 100 bands are detected when screening a genomic library under the same conditions.
References

Tso JY, et al. Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: Genomic complexity and molecular evolution of the gene. Nucleic Acids Res. 13: 2485-2502, 1985. PubMed: 2987855

Norgard MV, et al. Activation of human monocytic cells by Treponema pallidum and Borrelia burgdorferi lipoproteins and synthetic lipopeptides proceeds via a pathway distinct from that of lipopolysaccharide but involves the transcriptional activator NF-kappaB. Infect. Immun. 64: 3845-3852, 1996. PubMed: 8751937

Filler SG, et al. Candida albicans stimulates cytokine production and leukocyte adhesion molecule expression by endothelial cells. Infect. Immun. 64: 2609-2617, 1996. PubMed: 8698486

Barbara Weiffenbach, personal communication

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