pHcGAP (ATCC® 57091)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

View Permits

Designations pHcGAP
GenBank Number


Species Homo sapiens, human
standard for analysis of gene expression glyceraldehyde-3-phosphate dehydrogenase glyceraldehyde 3-phosphate dehydrogenase, phosphoglyceraldehyde dehydrogenase, triosephosphate dehydrogenase
Construct size (kb): 5.599999904632568
Insert lengths(kb): 1.200000047683716
Tissue: fetal liver
Gene product: glyceraldehyde-3-phosphate dehydrogenase(glyceraldehyde 3-phosphate dehydrogenase, phosphoglyceraldehyde dehydrogenase, triosephosphate dehydrogenase) [GAPD]
Target Gene: glyceraldehyde-3-phosphate dehydrogenase
Insert Size (kb) 1.200
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
The PstI site at the 3' end of the insert was destroyed during cloning. The retained PstI site is 750 bp from the EcoRI site. The insert contains a HindIII and a XbaI site.
Restriction digests of the clone give the following sizes (kb): PstI--5.55; HindIII--4.5, 1.06; RsaI--2.1, 1.4, 0.86, 0.65, 0.56; XbaI/PstI--4.7, 0.8; BglI--2.9, 2.2, 0.4, 0.2; BglI/PstI--2.2, 1.8, 1.0, 0.4, 0.2.
Includes 31 bp of 5' and 216 bp of 3' untranslated sequence, and a complete 1008 bp coding sequence. The polyadenylation signal AATAA is 15 bp upstream from poly(A) site.
The XbaI site is about 780 bp from the 5' PstI site. A PstI/XbaI double digest should give a probe containing the 5' non-coding sequences and sequences encoding about the first 250 amino acids.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
Thirty hybridizing bands are detected in genomic blots at 37C, 50% formamide, 5X SSC; 100 bands are detected when screening a genomic library under the same conditions.

Tso JY, et al. Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: Genomic complexity and molecular evolution of the gene. Nucleic Acids Res. 13: 2485-2502, 1985. PubMed: 2987855

Norgard MV, et al. Activation of human monocytic cells by Treponema pallidum and Borrelia burgdorferi lipoproteins and synthetic lipopeptides proceeds via a pathway distinct from that of lipopolysaccharide but involves the transcriptional activator NF-kappaB. Infect. Immun. 64: 3845-3852, 1996. PubMed: 8751937

Filler SG, et al. Candida albicans stimulates cytokine production and leukocyte adhesion molecule expression by endothelial cells. Infect. Immun. 64: 2609-2617, 1996. PubMed: 8698486

Barbara Weiffenbach, personal communication

Product Sheet
Product Sheet
Product Sheet