45.1 DQbeta 021 (ATCC® 57082)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: EO Long

Permits and Restrictions

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Designations 45.1 DQbeta 021
Species Homo sapiens, human
Depositors EO Long
Construct size (kb): 4.099999904632568
Insert lengths(kb): 1.289999961853027
Tissue: hemizygous 45.1 cell line (DR1, DQw1, DPw2)
Gene product: major histocompatibility complex, class II, DQ beta 1(major histocompatibility complex, class II, lymphocyte antigen DQB) [HLA-DQB]
Insert Size (kb) 1.290
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Restriction digests of the clone give the following sizes (kb): BamHI--2.7, 0.9, 0.41, 0.26; EcoRI--4.1; HindIII--4.1; SalI--3.8, 0.46; PstI--2.7, 1.2, 0.56; PstI/XhoI--1.8, 1.2, 0.9, 0.56.
The 5' end of the insert is within the TATA sequence. The insert defines the complete sequence of HLA-DWw1 beta. PstI cuts within the insert.

Tonnelle C, et al. DObeta: a new beta chain gene in HLA-D with a distinct regulation of expression. EMBO J. 4: 2839-2847, 1985. PubMed: 2998758

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