HPV-11 plasmid in E. coli (ATCC® 45151)

Organism: human papilloma virus type 11  /  Clone Type: Clone  /  Depositors: E de Villiers, H zur Hausen

Permits and Restrictions

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Designations HPV-11 plasmid in E. coli
GenBank Number

M14119

Species human papilloma virus type 11
Depositors E de Villiers, H zur Hausen
Vector
Construct size (kb): 12.30
Insert site: BamHI
Excise insert: BamHI
Insert
DNA: genomic
Insert lengths(kb): 7.931
Tissue: laryngeal papilloma
Gene product: human papilloma virus genome
Insert ends: BamHI
Insert Size (kb) 7.931
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products
Purified plasmid DNA: 45151D
Biosafety Level 1
Shipping Information

Shipped:  Frozen glycerol stock of Escherichia coli HB101 containing the plasmid.

Comments
Full-length genome of pHPV-11 cloned into BamH1 site of pBR322. Note: The depositor has requested that this deposit be distributed exclusively for scientific and not for commercial purposes.
Constructed by linearizing the virus at the unique BamHI site (nt 7072).
Cloned from human papilloma virus type 11 laryngeal papilloma
Restriction digests of the clone give the following sizes (kb): BamHI--7.9, 4.4; HindIII--9.4, 2.9; PstI--4.7, 1.8, 1.5, 1.3, 1.1, 0.8 (doublet), 0.45.
Classification Papovavirus, Papillomavirus, recombinant
References

Dartmann K, et al. The nucleotide sequence and genome organization of human papilloma virus type 11. Virology 151: 124-130, 1986. PubMed: 3008427

Harald zur Hausen, personal communication

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