pWS9 (ATCC® 45087)

Organism: Wound tumor virus  /  Clone Type: Clone  /  Depositors: DL Nuss

Permits and Restrictions

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Designations pWS9
GenBank Number

M24115

Species Wound tumor virus
Depositors DL Nuss
Vector
Construct size (kb): 4.050000190734863
Insert
DNA: cDNA
Insert lengths(kb): 1.179999947547913
Gene product: segment S9, Pns10 polypeptide
Insert Size (kb) 1.180
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
The insert contains internal AvaI, XbaI, HincII and HindIII sites.
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 1.2; EcoRI--4.1; HindIII--3.3, 0.84; PstI--4.1; XbaI--4.0, 0.13.
Linearize the plasmid with SalI for in vitro transcription.
This is a full-length clone of virus segment S9.
The cDNA ends were tailored to terminal BamHI sites before cloning into the BamHI site of pGEM-1.
Classification Phytoreovirus
References

Xu ZK, et al. Assignment of wound tumor virus nonstructural polypeptides to cognate dsRNA genome segments by in vitro expression of tailored full-length cDNA clones. Virology 168: 73-78, 1989. PubMed: 2909991

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