pWS9 (ATCC® 45087)

Organism: Wound tumor virus  /  Clone Type: Clone  /  Depositors: DL Nuss

Permits and Restrictions

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Designations pWS9
GenBank Number

M24115

Species Wound tumor virus
Depositors DL Nuss
Vector
Construct size (kb): 4.050000190734863
Insert
DNA: cDNA
Insert lengths(kb): 1.179999947547913
Gene product: segment S9, Pns10 polypeptide
Insert Size (kb) 1.180
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
The insert contains internal AvaI, XbaI, HincII and HindIII sites.
Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 1.2; EcoRI--4.1; HindIII--3.3, 0.84; PstI--4.1; XbaI--4.0, 0.13.
Linearize the plasmid with SalI for in vitro transcription.
This is a full-length clone of virus segment S9.
The cDNA ends were tailored to terminal BamHI sites before cloning into the BamHI site of pGEM-1.
Classification Phytoreovirus
References

Xu ZK, et al. Assignment of wound tumor virus nonstructural polypeptides to cognate dsRNA genome segments by in vitro expression of tailored full-length cDNA clones. Virology 168: 73-78, 1989. PubMed: 2909991

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