pTL-0059 (ATCC® 45035)

Organism: Tobacco etch virus  /  Clone Type: Clone  /  Depositors: WG Dougherty

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Designations pTL-0059
Species Tobacco etch virus
Depositors WG Dougherty
Construct size (kb): 8.989999771118164
Insert lengths(kb): 5.909999847412109
Gene product: VPg, 6 kDa
Insert Size (kb) 5.910
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Restriction digests of the clone give the following sizes (kb): BamHI--9.0; PstI--9.0; HindIII--5.2, 2.25, 1.1 + others; EcoRI--9.0.
The vector (pTL-8) allows in vitro expression of the insert as a fusion protein.
The insert includes TEV cDNA sequences from nt 0 to 5915.
Classification Potyvirus

Allison R, et al. The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154: 9-20, 1986.

Carrington JC, et al. Vectors for cell-free expression and mutagenesis of protein-coding sequences. Nucleic Acids Res. 15: 10066, 1987. PubMed: 3697076

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