pBKV (34-2) plasmid in E. coli (ATCC® 45025)

Organism: BK polyomavirus, BK Virus  /  Clone Type: Clone  /  Depositors: PM Howley

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Designations pBKV (34-2) plasmid in E. coli
GenBank Definition

Human polomavirus BK nuclear factor.

GenBank Number

J02038

M28533

Species BK polyomavirus, BK Virus
Depositors PM Howley
Vector

Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction:
pBR313
Host range: Escherichia coli

 
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR

 
Cross references: DNA Seq. Acc.: J01749

Insert Genome: BK virus
Gene name: genome, full-length
Contains complete coding sequence?: U
Strain: Dunlop
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 5.0
Cross references: DNA Seq. Acc.: J02038
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5, 4.4; EcoRI--5.3, 4.1.
pBKV (34-2) from P. Howley
Circular BKV was linearized with BamHI before cloning.
Classification Polyomaviridae, Polyomavirus
References

Seif I, et al. The Genome of human papovavirus BKV. Cell 18: 963-977, 1979. PubMed: 229976

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