pBKV (34-2) plasmid in E. coli (ATCC® 45025)

Organism: BK polyomavirus, BK Virus  /  Clone Type: Clone  /  Depositors: PM Howley

Permits and Restrictions

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Designations pBKV (34-2) plasmid in E. coli
GenBank Definition

Human polomavirus BK nuclear factor.

GenBank Number

J02038

M28533

Species BK polyomavirus, BK Virus
Depositors PM Howley
Vector

Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction:
pBR313
Host range: Escherichia coli

 
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR

 
Cross references: DNA Seq. Acc.: J01749

Insert Genome: BK virus
Gene name: genome, full-length
Contains complete coding sequence?: U
Strain: Dunlop
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 5.0
Cross references: DNA Seq. Acc.: J02038
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5, 4.4; EcoRI--5.3, 4.1.
pBKV (34-2) from P. Howley
Circular BKV was linearized with BamHI before cloning.
Classification Polyomaviridae, Polyomavirus
References

Seif I, et al. The Genome of human papovavirus BKV. Cell 18: 963-977, 1979. PubMed: 229976

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