pSRA-2 (ATCC® 45000)

Organism: Rous sarcoma virus  /  Clone Type: Clone  /  Depositors: J Bishop

Permits and Restrictions

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Designations pSRA-2
Species Rous sarcoma virus
Depositors J Bishop
Vector
Construct size (kb): 13.69999980926514
Insert
DNA: genomic
Insert lengths(kb): 9.399999618530273
Tissue: DNA from quail QT-6 cell line
Insert Size (kb) 9.400
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.5, 4.3, 1.7; EcoRI--8.2, 3.0, 2.5; SalI--9.4, 4.3.
The clone was derived from unintegrated DNA from an acute infection of QT-6 cells.
Unintegrated DNA isolated from an acute infection of QT-6 cells by SRA-2 virus. Permuted SRA-2 molecule from lambda gt WES was circul
A gag-specific probe can be generated from a 1.35 kb BamHI fragment; a pol-specific probe can be generated from a 0.8 kb HindIII/BamHI fragment; an LTR-specific probe from a 0.32 kb EcoRI fragment, and a src-specific probe from a 0.8 kb PvuII fragment.
SRA-2 from lambdagtWES (linearized at SacI) was circularized, cut with SalI, and cloned into pBR322.
Classification Retrovirus, Avian Type C Retrovirus Group
References

DeLorbe WJ, et al. Molecular cloning and characterization of avian sarcoma virus circular DNA molecules. J. Virol. 36: 50-61, 1980. PubMed: 6255214

J. Michael Bishop, personal communication

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