pSRA-2 (ATCC® 45000)

Organism: Rous sarcoma virus  /  Clone Type: Clone  /  Depositors: J Bishop

Permits and Restrictions

View Permits

Designations pSRA-2
Species Rous sarcoma virus
Depositors J Bishop
Vector
Construct size (kb): 13.69999980926514
Insert
DNA: genomic
Insert lengths(kb): 9.399999618530273
Tissue: DNA from quail QT-6 cell line
Insert Size (kb) 9.400
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.5, 4.3, 1.7; EcoRI--8.2, 3.0, 2.5; SalI--9.4, 4.3.
The clone was derived from unintegrated DNA from an acute infection of QT-6 cells.
Unintegrated DNA isolated from an acute infection of QT-6 cells by SRA-2 virus. Permuted SRA-2 molecule from lambda gt WES was circul
A gag-specific probe can be generated from a 1.35 kb BamHI fragment; a pol-specific probe can be generated from a 0.8 kb HindIII/BamHI fragment; an LTR-specific probe from a 0.32 kb EcoRI fragment, and a src-specific probe from a 0.8 kb PvuII fragment.
SRA-2 from lambdagtWES (linearized at SacI) was circularized, cut with SalI, and cloned into pBR322.
Classification Retrovirus, Avian Type C Retrovirus Group
References

DeLorbe WJ, et al. Molecular cloning and characterization of avian sarcoma virus circular DNA molecules. J. Virol. 36: 50-61, 1980. PubMed: 6255214

J. Michael Bishop, personal communication

Product Sheet
Product Sheet
Product Sheet