NEB619 (ATCC® 40795)

Organism: Thermococcus litoralis Neuner et al.  /  Clone Type: Clone  /  Depositors: New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.

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Designations NEB619
GenBank Number

M74198

Species Thermococcus litoralis Neuner et al.
Depositors New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.
Vector
Construct size (kb): 43.0
Insert
DNA: genomic
Insert lengths(kb): 14.0
Gene product: DNA polymerase (Vent) [pol]
Insert Size (kb) 14.0
Media Medium 1592: SM buffer
Biosafety Level 1
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 14.0, 9.4; BglII--23.0, 7.0, 4.8, 3.6, 2.6, 0.9, 0.65; EcoRI--20.0, 9.4, 2.9, 2.1, 1.9, 1.7, 1.6, 1.3 (doublet), 1.1, 0.65;
HindIII--21.0, 11.0, 2.7, 2.2, 1.5, 1.15, 1.05, 1.0, 0.7 (doublet); SacI--20.0, 9.4, 4.8, 2.2, 1.6 (doublet), 1.3, 1.05, 0.9, 0.65, 0.5.
Contains the complete coding sequence within a 8.0 kb XbaI fragment.
References

Comb DG, Kucera RB. Purified thermostable DNA polymerase obtainable from Thermococcus litoralis. US Patent 5,210,036 dated May 11 1993

Comb DG, et al. Purified thermostable DNA polymerase obtainable from Thermococcus litoralis. US Patent 5,322,785 dated Jun 21 1994

Comb DG, et al. Recombinant thermostable DNA polymerase from archaebacteria. US Patent 5,352,778 dated Oct 4 1994

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