lambdaHP2-4 (ATCC® 40700)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: CM Croce

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Designations lambdaHP2-4
Species Homo sapiens, human
Depositors CM Croce
Vector
Construct size (kb): 42.79999923706055
Insert
DNA: genomic
Insert lengths(kb): 13.80000019073486
Tissue: placenta
Gene product: B cell CLL/lymphoma 5 [BCL5]
Insert Size (kb) 13.800
Biosafety Level 1
Comments
Restriction digests of the clone give the following sizes (kb): SalI--20.0, 14.0, 9.0; EcoRI--26.0, 12.0, 3.8; HindIII--22.0, 14.5, 4.5, 2.5; SacI--21.0, 13.0, 4.6, 2.7, 1.1, 0.65; SmaI--19.5, 10.5, 6.0, 2.8, 2.4, 1.6, 0.8, 0.45.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--2.9, 7.9, 10.6, 11.6, 12.2; HindIII--9.3, 11.5; EcoRI--3.4, 7.9.
The 5.0 SacI fragment includes the breakpoint region in t(18,17) rearrangements involving c-myc.
One of two overlapping clones from a normal chromosome 17. It includes the breakpoint region of some t(8;17) translocations.
LambdaHP4-4 extends farther upstream in the 5' flanking region and overlaps lambdaHP2-4 by about 9.3 kb. These clones share a 5.0 kb SacI fragment and a 4.5 kb EcoRI fragment that contain the promoter region and some expressed sequence.
The 5.0 kb fragment detects a 1.7 kb transcript in human hematopoietic cells.
References

Gauwerky CE, et al. Activation of MYC in a masked t(8;17) translocation results in an aggressive B-cell leukemia. Proc. Natl. Acad. Sci. USA 86: 8867-8871, 1989. PubMed: 2682663

Croce CM. Methods for detecting Bcl-3 gene in human leukemias. US Patent 5,149,628 dated Sep 22 1992

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