pGH202 plasmid in Streptomyces lividans (ATCC® 39896)

Applications: produces protein endo-beta-N-acetylglucosaminidaseshuttle vectorvector with sequences for protein export endo-beta-N-acetylglucosaminidase  /  Depositors: OmniGene, Inc., S Guterman, OmniGene, Inc.

Designations pGH202 plasmid in Streptomyces lividans
Depositors OmniGene, Inc., S Guterman, OmniGene, Inc.
Biosafety Level 1
Host
Distribution host: Streptomyces lividans
Vector Information
Size (kb): 11.30
Vector: pGH202 (plasmid)
Construction: pEHB1.6 pIJ702
Marker(s):ampR tsrR
Construct size (kb): 11.30
Features: marker(s): ampR
marker(s): tsrR
replicon: pIJ101
replicon: pMB1
signal peptide coding region: endo H
coding sequence: endo H
Applications
produces protein endo-beta-N-acetylglucosaminidase
shuttle vector
vector with sequences for protein export endo-beta-N-acetylglucosaminidase
Comments
Shuttle expression vector permitting secretion of fusion proteins from S. lividans hosts.
pGH202 (ATCC 39896) and pGH205 (ATCC 39895) differ in that the latter includes a promoter element for aminoglycoside phosphotransferase (aph).
S. lividans transformed with this plasmid will secrete endo H when grown in this medium: Difco yeast extract (3 g/L), Difco peptone (5 g/L), Oxoid malt extract (3 g/L), glucose (10 g/L), sucrose (340 g/L), and 5 mM MgCl2.
Media YEME-thiostrepton medium
Growth Conditions
Temperature: 30.0°C
References

Guterman S. Streptomyces secretion vector. US Patent 4,745,056 dated May 17 1988

Robbins PW, et al. Primary structure of the Streptomyces enzyme endo-beta-N-acetylglucosamine. J. Biol. Chem. 259: 7577-7583, 1984. PubMed: 6429133

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet