pKB766 (ATCC® 39858)

Applications: promoter-cloning vector  /  Depositors: OmniGene, Inc., KC Backman, OmniGene, Inc.

Permits and Restrictions

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Designations pKB766
Depositors OmniGene, Inc., KC Backman, OmniGene, Inc.
Biosafety Level 1
Distribution host: Escherichia coli YMC9 (ATCC 33927)
Vector Information
Size (kb): 3.5000000000000000
Name of vector: pKB678
Intact vector size: 4.360
Type of vector: plasmid
Vector end: KpnI
Vector end: EcoRI
Cloning sites: EcoRI KpnI
Polylinker sites:
Construction: pBR322
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references:
Vector: pKB766 (plasmid)
Construction: pBR322, pheA
Construct size (kb): 3.5
Features: marker(s): ampR
replicon: pMB1
restriction site: BglII
restriction site: EcoRI
coding sequence: pheA
promoter-cloning vector
Restriction digests of the clone give the following sizes (kb): EcoRI/BglII--1.2, 2.2; EcoRI--3.5; NcoI--3.5.
Promoter cloning vector containing the complete coding sequence for pheA. Expression can be determined by testing for secretion of phenylalanine and cross-feeding of phenylalanine auxotrophs.
The construct contains a weak attenuator sequence at the 5' end of the pheA coding sequence.
Media ATCC® Medium 1315: LB Medium (ATCC medium 1065) with 100 mg/L ampicillin
Growth Conditions
Temperature: 37.0°C

Backman KC. Method of biosynthesis and cells therefor. US Patent 4,839,286 dated Jun 13 1989

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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