Escherichia coli (Migula) Castellani and Chalmers (ATCC® 39625)

Strain Designations: SA431 (lambdabio10cI857O/P-dQ-J) [211, SA431 O/P-d, SA431(lambdabio10cI857O/P-d)]  /  Type Strain: no  /  Biosafety Level: 1

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Classification Enterobacteriaceae, Escherichia
Strain Designations SA431 (lambdabio10cI857O/P-dQ-J) [211, SA431 O/P-d, SA431(lambdabio10cI857O/P-d)]
Application
transformation host
Type Strain no
Biosafety Level 1
Product Format freeze-dried
Shipping Information Distributed: freeze-dried
Preceptrol® no
Genotype F- uvrB chlD lambda bio10 cI857 O/P-d Q-J
Type Strain no
Comments
An Escherichia coli K-12 bio+ strain, immune to lambda, surviving cultivation at 42C, sensitive to lambda imm434 at 30C but immune at 42C.
Derived from SA431 which contains lambda deleted for genes O S R A J attR on the right and cI857 on the left.
An Escherichia coli K-12 bio+ strain, immune to lambda, surviving cultivation at 42C, sensitive to lambda imm434 at 30C but immune at 42C
An Escherichia coli lambda lysogen that continuously produces defective O and P genes above 39C
An Escherichia coli lambda lysogen that continuously produces defective O and P genes above 39C.
Derived from SA431 which contains lambda deleted for genes O S R A J attR on the right and cI857 on the left.
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 30.0°C
Genotype F- uvrB chlD lambda bio10 cI857 O/P-d Q-J
Name of Depositor Wisconsin Alumni Res. Fndn., WT Szybalski, Wisconsin Alumni Res. Fndn.
References

Szybalski WT. Partially defective foreign gene for conferring immunity on a biological host. US Patent 4,774,182 dated Sep 27 1988

Waclaw T Szybalski, personal communication

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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