Mtchi5 (ATCC® 39275)

Applications: YE-type (episomal) shuttle vectorshuttle vector  /  Depositors: Sandoz Crop Protection, PA Okubara, Sandoz Crop Protection

Designations Mtchi5
Depositors Sandoz Crop Protection, PA Okubara, Sandoz Crop Protection
Biosafety Level 1
Host
Distribution host: Escherichia coli LE392
Vector Information
Size (kb): 10.6999998092651400
Vector: Mtchi5 (plasmid)
Construction: YEp13, pFE364
Marker(s):LEU2,ampR,DHFR
Construct size (kb): 10.69999980926514
Features: marker(s): ampR, DHFR, LEU2
replicon: pMB1, 2 micron
Applications
YE-type (episomal) shuttle vector
shuttle vector
Comments
This vector contains the following sites separated by (bp): Sau3A I- 130- BamHI- 280- SalI- 3700- EcoRI- 2230- EcoRI- 860- SalI- 3250- HindIII- 255- Sau3A I.
Restriction digests of the clone give the following sizes (kb): BamHI--11.0; HindIII--11.0; EcoRI--11.5.
An episomal Saccharomyces cerevisiae/Escherichia coli shuttle vector.
A 370 bp HaeIII/AluI fragment encoding dihydrofolate reductase (ends filled, BamHI and HindIII linkers added) was inserted between the BamHI and HindIII sites of YEp13. DHFR transcription runs from BamHI toward the HindIII site.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Okubara PA, et al. Hybrid plasmid with marker. US Patent 4,670,399 dated Jun 2 1987

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet